Table I

Morphology of Transfected N1E-115 Cells

RoundedNeurite-bearing*
%%
Serum-free:
Control11 ± 130 ± 4
V14RhoA33 ± 313 ± 2
RhoGEF33 ± 217 ± 2
ΔRhoDEF‡34 ± 614 ± 2
8% serum:
Control54 ± 4 5 ± 2
N19RhoA24 ± 534 ± 2
p116Rip§38 ± 520 ± 2
Δp116Rip56 ± 10 9 ± 3
  • N1E-115 cells were transiently transfected with the indicated plasmids together with the LacZ gene to identify transfected cells, as described in Materials and Methods. Cell morphology was assessed in both serum-free medium (which promotes neurite outgrowth) and serum-containing medium (which contains LPA and prevents neurite outgrowth), as indicated. The percentage of undifferentiated (rounded without neurites) and neurite-bearing cells (flattened) was calculated from at least 300 positive cells counted. An average percentage was calculated from three dishes per transfection and three independent transfections. Numbers are percentages of total number of blue-stained cells (mean ± SEM). Differences with control cells are statistically significant (P < 0.001) according to t test. For details see Materials and Methods.  

  • *  Neurites longer than twice the soma diameter were scored positive.  

  •  ΔRhoGEF lacks amino acids 1–684.  

  • §  Δp116Rip lacks amino acids 1–544.