Table I

Purification Scheme for Cofactor B

DimensionMatrixSize*:Buffer‡Activity‖
1Q-Sepharose HP5/25:I/II80–100 mM
2Hydroxylapatite2.6/12:III/IVnonbinding
3MonoQ1/10:XIV/XV0 .22–0.24 M
4Q-Sepharose HP1/10:XIX/XX§0 .25–0.26 M
5TSKG3000SW0.75/60:X
  • *  Size of column bed (width/height) in cm.  

  •  Buffer composition as described in Tian et al. (1996)  

  • §  The composition of buffers used in this dimension was XIX: 20 mM diethanolamine-HCl, 20 mM KCl, 1 mM MgCl2, 1 mM EGTA, 1 mM DTT; XX: same as XIX, except containing 1 M in place of 20 mM KCl.  

  •  Counterion concentration containing cofactor B activity.