Table I

Diffusion Constants (D) and Immobile Fractions of LBR–GFP

MembraneCell cycle stageDImmobile fraction (%)
μm2/s
ERinterphase0.41 ± 0.1*3.1 ± 3.9*
ERmitosis0.38 ± 0.1*‡6.4 ± 7.7*‡
NEinterphaseNA§>60
NEtelophaseNA§>55
  • *  Standard deviations are given from a mean of n ⩾7 independent determinations.  

  •  Not significantly different from ER in interphase as determined by t test, P > 0.5.  

  • §  Not applicable, as immobile fraction rose above 55% and kinetics did not fit a diffusional profile.