Table I

Test for Presence of Plastid-localized Proteins in Δycf3 Plants by Immunoblot Analyses

GeneGene LocalizationGene ProductDetectable in Δycf3 Plants
aptBPlastidATP synthase CF1 β subunitYes
psbAPlastidD1 protein of PSIIYes
psbDPlastidD2 protein of PSIIYes
PsbONucleusPsbO, manganese-stabilizing  protein of PSIIYes
PsbPNucleusPsbP, lumen-localized extrinsic  protein of PSIIYes
Lchb6Nucleus20-kD apoprotein of CP24Yes
petAPlastidCytochrome fYes
PetENucleusPlastocyaninYes
psaCPlastidPsaC, subunit VII of PSI,  FA/FB-binding proteinNo
PsaDNucleusPsaD, subunit, II of PSI,  ferredoxin-binding proteinNo
PsaFNucleusPsaF, subunit III of PSI,  plastocyanin-docking proteinNo
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