Table I

Micromolar Dissociation Constants (Kd) for HA of each CD44Rg Glycovariant

Cell lineMedia additionN-GlycansO-GlycansKd± SDEnzyme treatmentN-GlycansO-GlycansKd
μMμM
CHOIntactIntact51± 19PNG-FAbsentIntact76
ldl-DGal/GalNAcIntactIntact45± 4PNG-FAbsentIntact62
ldl-DGalIntactAbsent47± 13PNG-FAbsentAbsent>150
ldl-DNo AdditionTruncatedAbsent 8± 1PNG-FAbsentAbsent>150
ldl-DGalNAcTruncatedTruncated 8± 2PNG-FAbsentTruncated112
CHOdMM/KIFHighIntact12Endo HGlcNAcIntact6
 mannose
ldl-DdMM/KIFHighAbsent10Endo HGlcNAcAbsent5
 mannose
CHOSwainsonineHybridIntact22
CHONANaseAsialo-Present12
CHONANase/Asialo-Present12
GALaseAgalacto-
CHONANase/TrimmedPresent9
GALase/
HEXase
ldl-DGal/GalNAcNANaseAsialo-Present13
ldl-DGal/GalNAcNANase/TrimmedPresent13
GALase/
HEXase
ldl-DGalNANaseAsialo-Absent18
ldl-DGalNANaseAsialo-Absent14
GALaseAgalacto-
ldl-DGalNANase/TrimmedAbsent14
GALase/
HEXase
  • Summary table of the micromolar dissociation constant (Kd) for each CD44Rg glycovariant obtained from the mean of two to seven ACE experiments. The standard deviation of the mean Kd is shown for values obtained from four or more experiments. PNG-F, peptide N-glycanase F; NANase, α-neuraminidase; GALase, β-galactosidase; HEXase, β-N-acetyl-hexosaminidase.