Table I

Genotypes of Yeast Strains and Plasmid Descriptions

StrainGenotypeSource
SEY6210MATα ura3-52 his3-Δ200 trp1-Δ901 leu2-3, 112 lys2-801 suc2-Δ9Emr lab strain collection
SEY6210aMATa ura3-52 his3-Δ200 trp1-Δ901 leu2-3, 112 lys2-801 suc2-Δ9Emr lab strain collection
SEY6210aMATα ura3-52 his3-Δ200 trp1-Δ901 leu2-3, 112 lys2-801 suc2-Δ9Emr lab strain collection
MATa ura3-52 his3-Δ200 trp1-Δ901 leu2-3, 112 lys2-801 suc2-Δ9
BWY20MATa pan1-20 ura3-52 his3-Δ200 trp1-Δ901 leu2-3, 112 lys2-801 suc2-Δ9This study
ΔSJL2/ΔSJL3MATα ura3-52 his3-Δ200 trp1-Δ63 leu2-Δ1 lys2-801 ade2-101 SJL2::HIS3 SJL3::TRP1Srinivasan et al., 1997
BWY236MATa pan1-20 ura3-52 his3-Δ200 trp1-Δ901 leu2-3, 112 lys2-801 suc2-Δ9This study
BWY249MATa pan1-20 ura3-52 his3-Δ200 trp1-Δ901 leu2-3, 112 lys2-801 suc2-Δ9 sjl1::HIS3 [pPAN1/416]This study
BWY250MATα ura3-52 his3-Δ200 trp1-Δ901 leu2-3, 112 lys2-801 suc2-Δ9 yap1801::HIS3This study
BWY256MATα ura3-52 his3-Δ200 trp1-Δ901 leu2-3, 112 lys2-801 suc2-Δ9 yap1802::LEU2This study
BWY255MATα ura3-52 his3-Δ200 trp1-Δ901 leu2-3, 112 lys2-801 suc2-Δ9 yap1801::HIS3 yap1802::LEU2This study
HF7cMATa ura3-52 his3-Δ200 trp1-Δ901 leu2-3, 112 lys2-801 ade2-101 gal4-542 gal80-538Clonetech, Stonybrook, NY
LYS2::GAL1-HIS3 URA3::(GAL4 17mers)3 -CYC1-lacZ
SFY526MATa ura3-52 his3-Δ200 trp1-Δ901 leu2-3, 112 lys2-801 ade2-101 canr gal4-542 gal80-538Clonetech
URA3::GAL1-lacZ
TVY614MATα ura3-52 his3-Δ200 trp1-Δ901 leu2-3, 112 lys2-801 suc2-Δ9 pep4::LEU2 prb1::HIS3 prc1::HIS3Emr lab strain collection
PSY15CMATα end1-1 ura3-52 his3-Δ200 trp1-Δ901 leu2-3, 112 lys2-801 suc2-Δ9Emr lab strain collection
BWY237MATα ura3-52 his3-Δ200 trp1-Δ901 leu2-3, 112 lys2-801 suc2-Δ9 pan1-like(YBL047c)::HIS3This study
TGY413-6DMATα erg6(ise1) ura3-52 his3-Δ200 leu2-3, 112 lys2-801 suc2-Δ9Graham et al., 1993
PlasmidDescriptionConstruction
pPAN1PAN1XmnI-DraI fragment in pRS415Wendland et al., 1996
pPAN1.1Pan1p EH1-2 (96–713)MscI-ClaI fragment of pPAN1 in SmaI of pGBT9This study
pPAN1.2Pan1p EH1 (96–403)Drop out SpeI-SalI fragment of pPAN1.1This study
pPAN1.3Pan1p EH2 (96–145/403–713)Drop out StyI-SalI fragment of pPAN1.1This study
pPAN1.4Pan1p EH2 (544–713)NciI-ClaI of pPAN1 in BamHI of pGBT9This study
pPAN1.5Pan1p (96-1480)MscI-SalI (polylinker site) fragment of pPAN1 in SmaI of pGBT9This study
pBS(-RV-HII)cloning vectorpBS(KS)II- cut with EcoRV and HindII and religatedThis study
pYAP180AYAP1801EcoRI fragment in pBS(-RV-HII)This study
pYAP180A.TAYAP1801EcoRI fragment in pCR2.1 (TA cloning vector)This study
pYAP180A.1yAP180A (432–637)Isolated in two-hybrid screen, pGADGH vectorThis study
pYAP180A.2yAP180A (439–637)Isolated in two-hybrid screen, pGADGH vectorThis study
pYAP180A.3yAP180A (24–637)Blunt BamHI fragment of pYAP180A.TA in blunt BamHI of pGADGHThis study
pYAP180AΔYAP1801::HIS3HIS3 replacement of EcoRV-ClaI fragment of pYAP180AThis study
pYAP180BYAP1802HindIII fragment in pBS(KS)II-This study
pYAP180B.1yAP180B (429-568)DraI fragment of pYAP180B in SmaI of pGADGHThis study
pYAP180BΔYAP1802::LEU23′ HindII fragment of yAP180B in pBS(KS)II-, 5′ AvaI fragment into SmaI site.  LEU2 inserted betweenThis study
pGEX-KG-KANcloning vectorpGEX-KG vector with kanamycin resistance gene insertedThis study
pYAP180A.4GST-yAP180A (24–637)Blunt BamHI fragment of pYAP180A.TA in blunt EcoRI of pGEX-KG-KANThis study
pYAP180A.5GST-yAP180A N1pYAP180A.4 cut with Nsil and Xhol, blunted and religatedThis study
pYAP180A.6GST-yAP180A C1pYAP180A.4 cut with Smal and Nsil, blunted and religatedThis study
pYAP180A.7GST-yAP180A C2pYAP180A.6 cut with Sacl and religatedThis study
pYAP180A.8GST-yAP180A C3Blunt Sacl fragment of pYAP180A.7 into blunt Xbal of pGEX-KG-KANThis study
pYAP180A.9GST-yAP180A C4pYAP180A.8 cut with Sacl and Pstl, blunted and religatedThis study
pYAP180A.10GST-yAP180A C4Blunt Sacl=Pstl fragment of pYAP180A.8 into blunt EcoRI of pGEX-KG-KANThis study
pYAP180B.2GST-yAP180B C1Dral fragment of pYAP180B in Smal of pGEX-KG-KANThis study
pGOGFP/416cloning vectorGFP fusion cassette in pRS416C. G. Odorizzi, unpublished
pGOGFP/426cloning vectorGFP fusion cassette in pRS426Cowles et al., 1997
pYAP180A.11GFP-yAP180APCR-generated SalI-KpnI fragment in pGOGFP/416This study
pYAP180A.12GFP-yAP180APCR-generated SalI-KpnI fragment in pGOGFP/426This study
pYAP180B.3GFP-yAP180BPCR-generated SalI-KpnI fragment in pGOGFP/416This study
pYAP180B.4GFP-yAP180BPCR-generated SalI-KpnI fragment in pGOGFP/426This study
pMS1SJL1::HIS3PCR-generated 5′ and 3′ fragments in pBS(KS)II- with HIS3 inserted betweenM.N.J. Seaman, unpublished