Table II

Two-Hybrid Interactions between Pan1p and yAP180 Proteins

BaitPrey
pGADGH (vector)yAP180A 24–637yAP180A 432–637yAP180B 429–568
pGBT9 (vector)<1 <1 <1 <1
Pan1p EH1–2 (96–713)     8.3 ± 2.078 ± 2117 ± 75362 ± 67
Pan1p EH1 (96–403)<118 ± 7    6 ± 1      70 ± 9
Pan1p EH2 (96–145/  403–713)<123 ± 4   33 ± 4342 ± 15
Pan1p EH2 (544–713)<1     2 ± 0.4    4 ± 1158 ± 15
Pan1p 96–1480<1     2 ± 0.1    2 ± 0.2 16 ± 9
(Units β-galactosidase activity)
  • Units of β-galactosidase activity were determined in SFY526 cells transformed with the indicated pair of plasmids as described in Materials and Methods.