Table I

Yeast Strains

Strain*Genotype
RH448ura3 leu2 his4 lys2 bar1-1
RH1298ste2::LEU2 ura3 leu2 his4 bar1-1
RH1597end4-1 ura3 leu2 his4 bar1-1
RH1965end4::LEU2 ura3 leu2 his4 lys2 bar1-1
RH3162ste2::LEU2 ura3 leu2 his3 trp1 bar1-1
RH3180ura3::ste2-345Stop[URA3],same as RH3162
RH3181ura3::ste2-K337R,345Stop[URA3],same as RH3162
RH3333ura3::ste2-S331A,S338A,S339A,345Stop[URA3],  same as RH3162
RH3510ura3::ste2-S331E,S338E,S339E,345Stop[URA3],  same as RH3162
RH3511ura3::ste2-S331D,S338D,S339D,345Stop[URA3],  same as RH3162
RH3589yck1Δ yck2-2 ura3 leu2 his3 ade2 bar1-1
RH3687ura3::ste2-S331A,S338A,S339A[URA3], same as RH3162
RH3992ura3 leu2 his3 lys2 trp1 bar1-1
LHY636ura3::ste2-S331E,S338E,S339E,345Stop[URA3],  same as RH3162
LHY638ura3::ste2-345Stop[URA3], same as RH3162
LHY639ura3::ste2-S331A,S338A,S339A,345Stop[URA3],  same as RH3162
  • *  All strains listed are MATa.