Table II

Flagellar Levels of FLA10 and IFT Particle Polypeptides in fla Mutants

Cell lineFlagellar resorption at 33°CFLA10 epitopeIFT polypeptides
23°C33°C23°C33°C
wild typeNo loss++++++++
fla1t1/2 = 5 h++
fla8t1/2 = 2 h±+
fla10-1t1/2 = 3 h±+
fla2t1/2 = 0.5 h+++++++++++
fla3t1/2 = 0.75 h+++++++/+
fla4t1/2 > 6 h++++
fla9t1/2 > 6 h++++++++++
fla13t1/2 = 4.5 h++++++++++
  • t1/2 is the time of incubation at 33°C when the average flagellar length is 50% preincubation length.