Table I

Cell Cycle Progression in HeLa and PtK1 Cells Injected with Control Rabbit IgG or Anti-p55CD

Injection/cell phase injectednNo. of cells arrested at metaphaseDuration of metaphase in min (mean ± SD)Duration of anaphase in min (mean ± SD)Specific notes
HeLa
 control rabbit IgG‡100   15.2 ± 4.6      3.2 ± 0.9
  early/late prometaphase              
 anti-p55CDC‡19035.0 ± 30.7‖      5.7 ± 3.0
  early/late prometaphase       
PtK1
 control rabbit IgG320   10.8 ± 4.7      9.3 ± 4.0
  late prophase/early metaphase               3 arrested at early anaphase
 anti-p55CDC§311038.0 ± 20.7‖23.3 ± 13.1‖4 arrested at late anaphase
  late prophase/early metaphase5 without cytokinesis
  • *  8.0 mg/ml in KCl-buffer.  

  •  2.0 mg/ml in KCl-buffer.  

  • § 1.0-2.5 mg/ml in KCl- or Tris-buffer.  

  • P < 0.01.