Table II

Yeast Strains

StrainGenotype*Reference
CRY1MATaade2-1oc can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1R. Fuller (Stanford University, CA)
JGY46MATa/MATα ade2-1oc/ade2-1oc can1-100/can1-100 his3-(Geiser et al., 1991)
11,15/his3-11,15 leu2-3,112/leu2-3,112 trp1-1/trp1-1 ura3-1/ura3-1
EMY55-5DMATaade2-1oc ade3Δ-100 can1-100 cyh2r his3-11,15 leu2-3,112 lys2Δ::HIS3 trp1-1 ura3-1E. Muller (University of
 Washington, Seattle, WA)
EMY55-6BMATα ade2-1oc ade3Δ-100 can1-100 cyh2S his3-11,15 leu2-3,112 lys2Δ::HIS3 trp1-1 ura3-1E. Muller (University of
Washington)
MMY28MATaade2-1oc ade3Δ-100 can1-100 (S65T-GFP)–CMD1 his3-(Moser et al., 1997)
11,15 leu2-3,112 lys2Δ::HIS3 trp1-1 ura3-1
RSY1EMY55-5D X EMY55-6BThis study
RSY2MATa/MATα ade2-1oc/ade2-1oc ade3Δ-100/ade3Δ-100 can1-100/can1-100 cyh2r/cyh2SThis study
his3-11,15/his3-11,15 leu2-3,112/leu2-3,112 lys2Δ::HIS3/lys2Δ::HIS3 MYO2/myo2Δ::TRP1
trp1-1/-trp1-1 ura3-1/ura3-1
RSY2-60BMATaade2-1oc ade3 Δ-100 can1-100 his3-11,15 leu2-3,112This study
lys2Δ::HIS3 myo2Δ::TRP1 trp1-1 ura3-1 carrying plasmid pRS50
RSY21MATaade2-1oc can1-100 his3-11,15 leu2-3,112 MYO2-D6IQ trp1-1 ura3-1This study
RSY22MATα ade2-1oc can1-100 his3-11,15 leu2-3,112 MYO2-D6IQ trp1-1 ura3-1This study
RSY38MATa/MATα ade2-1oc/ade2-1oc ADE3/ade3Δ-100 can1-100/can1-100 CMD1/GFP–CMD1This study
his3-11,15/his3-11,15 leu2-3,112/leu2-3,112 MYO2/MYO2-Δ6IQ MYO4/myo4Δ::URA3
trp1-1/trp1-1 ura3-1/ura3-1
RSY38-9DMATaade2-1oc ade3Δ-100 can1-100 GFP–CMD1 his3-11,15This study
leu2-3112 lys2Δ::HIS3 MYO2-Δ6IQ trp1-1 ura3-1
RSY38-16AMATaade2-1oc ade3Δ-100 can1-100 GFP–CMD1 his3-11,15This study
leu2-3,112 lys2Δ::HIS3 myo4Δ::URA3 trp1-1 ura3-1
RSY38-16CMATα ade2-1oc ade3Δ-100 can1-100 his3-11,15 leu2-3,112This study
lys2Δ::HIS3 MYO2-Δ6IQ myo4Δ::URA3 trp1-1 ura3-1
RSY38-17CMATα ade2-1oc ade3Δ-100 can1-100 GFP–CMD1 his3-11,15This study
leu2-3,112 lys2Δ::HIS3 trp1-1 ura3-1
RSY105MATa/MATα ade2-1 oc/ade2-1 oc can1-100/can1-100 his3-This study
11,15/his3-11,15 leu2-3,112/leu2-3,112 MLC1/mcl1D::TRP1
trp1-1/trp1-1 ura3-1/ura3-1 carrying plasmid pRS289
RSY105-6AMATα ade2-1oc can1-100 his3-11,15 leu2-3,112 mcl1Δ::TRP1This study
trp1-1 ura3-1 carrying plasmid pRS289
RSY107RSY21 X RSY105-6A carrying plasmid pRS289This study
RSY112MATa/MATα ade2-1oc/ade2-1oc ade3Δ-100/ade3Δ-100 can1-100/can1-100 his3-11,15/his3-11,15This study
leu2-3,112/leu2-3,112 lys2Δ::HIS3/lys2Δ::HIS3 MLC1/mcl1Δ::TRP1
MYO2/myo2Δ::TRP1 trp1-1/trp1-1 ura3-1/ura3-1 carrying plasmid pRS289
  • *GFP–CMD1 encodes a fusion protein of green fluorescent protein and calmodulin.