Table II

Characterization of Actin Polarity Defects in act1-159 Strains

PolarizedPartially polarizedDepolarized
%%%
ACT193 7 0
act1-159403624
• Cells were grown at 25°C. Actin structures were observed by rhodamine-phalloidin staining. To assess actin polarity, only small-budded cells were counted. Terms are defined as follows. Polarized, the majority of actin patches were in the bud; Partially polarized, more than half of the patches were in the bud, but there were also many additional patches in the mother, or the patches polarized to the neck instead of the bud; Depolarized, actin patch distribution was nearly random or the phalloidin staining structures were not easily identified as patches or cables.