Table I

Formation of Nuclear Inclusions by Truncated Ataxin-3 Containing Normal or Expanded Polyglutamine and a Nuclear Localization Signal

Construct transfected% transfected cells with NI
NLS-Q27-myc 75% (102/136)
NLS-Q78-myc82% (86/105)
  • HEK-293T cells were transfected with the above constructs and the percentage of transfected cells containing NI was quantified. Data were pooled from four independent experiments in which random fields were analyzed. The numbers in parentheses represent the ratio of inclusion positive cells to the total number of transfected cells counted.