Table I

Overexpression of Headless Xklp3 Decreases the Rate of Pigment Dispersion

Cell groupAverage half-times for dispersion and aggregation ± SD
DispersionAggregation
min
Nontransfected 7.0 ± 2.9‡19.0 ± 9.8*
pEGFP-C1 7.5 ± 1.4‡20.6 ± 10.8*
pEGFP-headless Xklp316.9 ± 7.1§22.8 ± 9.5*
Nocodazole-treated14.0 ± 4.7§ND
  • *  There is no significant difference between the values of half-times for aggregation for any two groups among them all. (Aggregation does not occur in the absence of microtubules, and therefore aggregation rates were not determined in nocodazole-treated cells.)  

  •  The half-times for dispersion for nontransfected cells and cells transfected with pEGFP-C1 are not significantly different.  

  • §  The half-time for dispersion for cells expressing headless Xklp3 or treated with nocodazole are significantly different from those obtained in nontransfected or EGFP-expressing cells (P < 0.01).