Table II

Overexpression of Headless Xklp3 Decreases the Frequency of Melanosome Saltations

Cell groupSaltations
PlusMinus
Nontransfected9163
pEGFP-C16247
pEGFP-headless Xklp33468
Colcemid-treated1312
  • Individual melanosomes were analyzed by video microscopy. 20 melanosomes were analyzed per cell, from three cells per treatment. Each melanosome was observed for a period for 5 min, during which the number of saltations was counted. The numbers per treatment are the total number of saltations in all three cells (i.e., 80 melanosomes).