Table IV

Frequency of Abnormal Anaphases in bub1 Mutants

StrainBackgroundNumber of late-anaphase cells analyzed*Late-anaphase cells with lagging chromosomes
 77Wild-type300      0
393Δbub1::ura4+40652 (12.8%)
277bub1-118404    38 (9.4%)
  • *  Late-anaphase cells were defined as cells with a spindle length greater than 5 μm.  

  •  Cells were classified as having lagging chromosomes when DAPI-stained material was observed on the spindle, at least 1.5 μm away from one spindle pole. Parentheses, fraction of anaphase cells with lagging chromosomes.