Table I

Yeast Strain List

Strain*Genotype
ProA5-4d/4aMATa/MATα NDC1-ProA-HIS3-URA3/NDC1-ProA-HIS3-URA3 ura3-52/ura3-52 his3Δ200/his3Δ200 trp1-1/trp1-1
leu2-3,112/leu2-3,112 lys2-801/lys2-801
ProA5-4dMATaNDC1-ProA-HIS3-URA3 ura3-52 his3Δ200 trp1-1 leu2-3,112 lys2-801
HC26-15a/1aMATa/MATα NDC1-ProA-HIS3-URA3/NDC1-ProA-HIS3-URA3 nup49-1::URA3/nup49-1::URA3 nup49ΔGFLG::GFP-
S65T-TRP1/nup49ΔGFLG::GFP-S65T-TRP1 ura3/ura3 his3/his3 trp1-1/trp1-1 leu2-3,112/leu2-3,112
HC27-16d/16bMATa/MATα nup133Δ::HIS3/nup133Δ::HIS3 NDC1-ProA-HIS3-URA3/NDC1 nup49-1::URA3/nup49-1::URA3
nup49ΔGFLG::GFP-S65T-TRP1/nup49ΔGFLG::GFP-S65T-TRP1 ura3/ura3 his3/his3 trp1-1/trp1-1 leu2-3,112/leu2-3,112
HC22-2b/1cMATa/MATα NDC1-ProA-HIS3-URA3/NDC1-ProA-HIS3-URA3 spc42Δ::LEU2/spc42Δ::LEU2
TRP1::SPC42-GFP(3x)/TRP1::SPC42-GFP(3x) ura3/ura3 his3/his3 trp1-1/trp1-1 leu2-3,112/leu2-3,112 ade2-1/ade2-1
HC23-11d/16aMATa/MATα NIC96-ProA-HIS3-URA3/NIC96-ProA-HIS3-URA3 spc42Δ::LEU2/spc42Δ::LEU2 TRP1::SPC42-
GFP(3x)/TRP1::SPC42-GFP(3x) ura3/ura3 his3/his3 trp1-1/trp1-1 leu2-3,112/leu2-3,112 ade2-1/ade2-1
HC14-10c(1198)/HC29-6bMATa/MATα ndc1Δ::KANMX/ndc1Δ::HIS3 ura3-52/ura3-52 his3Δ200/his3Δ200 trp1Δ63/TRP1 leu2Δ1/leu2Δ1 LYS2/
lys2-801 ade2Δ426/ade2Δ426 ade3Δ/ade3Δ (pRS315-NDC1-GFP)
HC10-42b/42cMATa/MATα ura3-52/ura3-52 ade2/ade2 his4-539/HIS4
HC10-42a/42dMATa/MATα ndc1-1/ndc1-1 ura3-52/ura3-52 ade2/ade2 his4-539/HIS4
HC13-1cMATα NIC96-ProA-HIS3-URA3 ura3-52 his3Δ200 trp1-1 leu2-3,112 lys2-801
Wx340-3cMATα ndc1-1 tub2-403 ura3-52 his4-539 lys2-801
Wx340-3dMATα tub2-403 ura3-52 his4-539
HC14-10cMATaura3-52 his3Δ200 trp1Δ63 leu2Δ1 ade2Δ426 ade3Δ
HC5-31cMATandc1-1 ura3-52 his3Δ200 trp1Δ63 leu2Δ1 ade2Δ426 ade3Δ
HC5-31c(1166)MATandc1-1 pom152Δ::HIS3 ura3-52 his3Δ200 trp1Δ63 leu2Δ1 ade2Δ426 ade3Δ
  • *  All strains were constructed as a part of this study.