Table I

Yeast Strains Used in This Study

W303MATa, ura3-52, his3-200, ade2-101, trp1-901, leu2-3,112
W303Δpex18MATa, ura3-52, his3-200, ade2-101, trp1-901, leu2-3,112, pex18::TRP1
W303Δpex21MATa, ura3-52, his3-200, ade2-101, trp1-901, leu2-3,112, pex21::URA3
W303Δpex18Δpex21*MATa, ura3-52, his3-200, ade2-101, trp1-901, leu2-3,112, pex18::TRP1, pex21::URA3
W303Δpot1MATa, ura3-52, his3-200, ade2-101, trp1-901, leu2-3,112, pot1::HIS3
PJ69-4AMATa, trp1-901, leu2-3,112, ura3-52, his3-200, gal4Δ, gal80Δ, GAL2-ADE2, LYS2::GAL1-HIS3, met2::GAL7-lacz
PJ69-4AΔpex7MATa, trp1-901, leu2-3,112, ura3-52, his3-200, gal4Δ, gal80Δ, GAL2-ADE2, LYS2::GAL1-HIS3, met2::GAL7-lacz, pex7::HIS3
PJ69-4AΔpot1MATa, trp1-901, leu2-3,112, ura3-52, his3-200, gal4Δ, gal80Δ, GAL2-ADE2, LYS2::GAL1-HIS3, met2::GAL7-lacz,
pot1::HIS3
PJ69-4AΔpex18Δpex21MATa, trp1-901, leu2-3,112, ura3-52, his3-200, gal4Δ, gal80Δ, GAL2-ADE2, LYS2::GAL1-HIS3,
met2::GAL7-lacz, pex18::TRP1, pex21::URA3
PJ69-4AΔpex13MATa, trp1-901, leu2-3,112, ura3-52, his3-200, gal4Δ, gal80Δ, GAL2-ADE2, LYS2::GAL1-HIS3,
met2::GAL7-lacz, pex13::URA3
PJ69-4AΔpex14MATa, trp1-901, leu2-3,112, ura3-52, his3-200, gal4Δ, gal80Δ, GAL2-ADE2, LYS2::GAL1-HIS3,
met2::GAL7-lacz, pex14::URA3
  • *  For expression of URA3 based plasmids in W303Δpex18Δpex21, the URA3 gene integrated at the PEX21 locus was first disrupted with HIS3, using the marker-swap plasmid pUH4 (Cross, 1997).