Table I

Yeast Strains Used in This Study

StrainGenotype
DBY1830a/αade2/+ lys2-801/+ his3-Δ200/his3-Δ200 ura3-52/ura3-52 leu2-3,112/leu2-3,112 trp1-1/trp1-1
CBY1830-53a/α ade2/+ lys2-801/+ his3-Δ200/his3-Δ200 ura3-52/ura3-52 leu2-3,112/leu2-3,112 trp1-1/trp1-1 sli15-Δ2::HIS3/+
CCY108-15C-1a ade2 his3-Δ200 ura3-52 lys2-Δ101::HIS3::lys2-Δ102 ipl1-2
CCY396-8Da ade2 ade3-130 lys2-801 ura3-52 leu2-3,112 his3-Δ200 ipl1-2 [IPL1, URA3, ADE3] (i.e., with the 2μ-plasmid pCC476)
CCY405-10Bα ade2 ade3-130 lys2-801 his3-Δ200 ura3-52 leu2-3,112 ipl1-2
CCY405-10B-2αade2 ade3-130 lys2-801 his3-Δ200 ura3-52 leu2-3,112 ipl1-2 LEU2 (at SLI15)
CCY482-13Daade2 his3-Δ200 ura3-52 leu2-3,112 lys2-Δ101::HIS3::lys2-Δ102
CCY482-13D-1-1aade2 his3-Δ200 ura3-52 leu2-3,112 lys2-Δ101::HIS3::lys2-Δ102 sli15-3
CCY598-49Cαade2 ade3-130 ura3-52 leu2-3,112 his3-Δ200 ipl1-2 LEU2 (at ipl1-2)
CCY598-52Baade2 ade3-130 ura3-52 leu2-3,112 his3-Δ200 ipl1-2 LEU2 (at ipl1-2)
CCY757-1Daade2 ade3-130 lys2-801 ura3-52 leu2-3,112 his3-Δ200 ipl1-2 sli15-13 [IPL1, URA3, ADE3] (i.e., with the 2μ-plasmid pCC476)
CCY766-9D-1alys2-801 his3-Δ200 ura3-52 leu2-3,112 trp1-1 NUF2::URA3-NUF2-SGFP
CCY822-6Bαade2 ade3-130 ura3-52 leu2-3,112 his3-Δ200 ipl1-2 sli15-1 [IPL1, URA3, ADE3] (i.e., with the 2μ-plasmid pCC476)
CCY822-7Baade2 ade3-130 ura3-52 leu2-3,112 his3-Δ200 ipl1-2 sli15-1 [IPL1, URA3, ADE3] (i.e., with the 2μ-plasmid pCC476)
CCY915-2Bαlys2-801 ura3-52 his3-Δ200 trp1-1 ipl1-2
CCY915-13C-5alys2-801 his3-Δ200 ura3-52 leu2-3,112 trp1-1 ipl1-2 NUF2::URA3-NUF2-SGFP
CCY941-2Cαlys2-801 ura3-52 leu2-3,112 his3-Δ200 ipl1-2 URA3 (at ipl1-2)
CCY1022-10Calys2-801 his3-Δ200 ura3-52 leu2-3,112 trp1-1 sli15-Δ2::HIS3 [URA3, SLI15] (i.e., with the CEN-plasmid pCC977)
CCY1060-1Daade2 ura3-52 leu2-3,112 his3-Δ200 lys2-Δ101::HIS3::lys2-Δ102 sli15-3
CCY1060-1D-4aade2 ura3-52 leu2-3,112 his3-Δ200 lys2-Δ101::HIS3::lys2-Δ102 sli15-3 LEU2 (at sli15-3)
CCY1076-28Bαlys2 his3 ura3 leu2 trp1-1 ura3::URA3-tetO112 leu2::LEU2-tetR-GFP
CCY1077-6Cαlys2 his3 ura3 leu2 ipl1-2 ura3::URA3-tetO112 leu2::LEU2-tetR-GFP
CCY1078-37Bαlys2 his3 ura3 leu2 sli15-3 ura3::URA3-tetO112 leu2::LEU2-tetR-GFP
CCY1083-8Balys2-801 his3-Δ200 ura3-52 leu2-3,112 sli15-3 NUF2::URA3-NUF2-SGFP
TD4aura3-52 leu2-3,112 trp1-289 his4-519
  • Most of the strains were constructed specifically for this study, the exceptions being DBY1830, which is from D. Botstein's laboratory collection (Stanford University, Palo Alto, CA), and TD4, which is from G. Fink's laboratory collection (Whitehead Institute, Cambridge, MA). The origin of some of the markers used is indicated in the text. Genes shown within square brackets are plasmid-borne.