Table 1

Drosophila Melanogaster Stocks Used in Epistasis Tests

GeneCross schemeResults
wgwgCX4/CyO; dAPC2ΔS females ×× malesAll wg progeny show the suppressed phenotype (n > 100).
wgCX4/CyO; dAPC2ΔS/Df(3R)crb87-4 females ××1/3 of wg progeny show the suppressed phenotype
wgCX4/+; +/Df(3R)crb87-4 males(n = 40, Df homozygotes show crumbs phenotype).
armarmH8.6/FM7/dAPC2ΔS females ×× dAPC2ΔS males1/4 of the progeny show the arm zygotic phenotype
(presumably armH8.6/Y; dAPC2ΔS male embryos).
3/4 show excess naked cuticle like dAPC2ΔS single mutants
(n > 200).
dTCFdAPC2ΔS; dTCF3/eyD females ×× males1/4 of the progeny show the dTCF zygotic phenotype, and
the remainder have excess naked cuticle (n > 200).
dsh1. dsh75 FRT101/FM7; dAPC2ΔS/TM6 females ××Zygotic loss of dsh does not modify the dAPC2ΔS mutant
ovoD1 FRT101/Y; hsp-FLP(F38); dAPC2ΔS malesphenotype.
2. Heat-shock progeny as third instar larvae twice to induce recombination
3. From among progeny cross:In the first (n > 30) and second (n = 23) crosses, 1/2 the
dsh75 FRT101/ovoD1 FRT101; dAPC2ΔS females ××progeny show the dsh germline clone mutant phenotype
1. dAPC2ΔS males,(presumed to be the dsh75/Y; dAPC2ΔS male embryos) and
2. dAPC2ΔS/Df(3R)crb87-4 males, or1/2 show a partially suppressed dAPC2ΔS mutant
3. Df(3R)crb87-4/TM3 malesphenotype, due to paternal rescue of dsh (n > 30). The partial
suppression of the dAPC2ΔS mutant phenotype is more
dramatic ventrally, with most denticle belts fully formed; the
dorsal surface is largely unrescued. In the cross to
Df(3R)crb87-4/TM3 males, some progeny hatch
(presumably those that are paternally wild-type), but dead
embryos show phenotypes identical to those in the other
crosses (n = 19).