Table 2

Plasmids Used in This Study

PlasmidCharacteristic and source
pGAL-BCK1*pPAD91, BCK1-20 allele under the control of the GAL1 promoter
pGAL-MKK1*pNV7-MKK1(P386), expresses activated-MKK1 under the control of the GAL1 promoter (Watanabe et al. 1995)
pGAL-PKC1*pBM743, expresses activated-PKC1 under the control of the GAL1 promoter (D.E. Levin)
pGAL-RHO1pTB230, expresses the functional NH2 terminally 2×HA-tagged RHO1 under the control of the GAL1 promoter
(Schmidt et al. 1997)
pGAL-RHO1*pAS106, expresses functional NH2 terminally HA-tagged activated RHO1 (H68) under the control of the GAL1 promoter
pGAL-RHO2pAS35, expresses functional NH2 terminally HA-tagged RHO2 under the control of the GAL1 promoter
pGAL-ROM2pAS48, expresses functional NH2 terminally HA-tagged ROM2 under the control of the GAL1 promoter
pGAL-WSC1pPAD62, WSC1 under the control of the GAL1 promoter
pGAL-WSC2pPAD45, expresses functional COOH terminally cmyc-tagged WSC2 under the control of the GAL1 promoter
pGAL-WSC3pPAD48, expresses functional COOH terminally cmyc-tagged WSC3 under the control of the GAL1 promoter
pHA-RHO1pAS97, expresses functional NH2 terminally HA-tagged RHO1 from its own promoter (CEN URA3)
pHAspPAD80/81/82/83, 3×HA tagging vector (see Materials and Methods),
LEU2, CEN LEU2, CEN URA3,URA3, respectively
pWSC1-HApPAD86, expresses functional COOH terminally 3×HA-tagged WSC1 from its own promoter (see Materials and Methods)