Table 2

Plasmids

NameTypeAuxotrophic markerOther featuresSource
pJP10-2Bcentromere/ARS (low copy number)URA3MYO2Johnston et al. 1991
pRS303MYO2integratingHIS3Integrates 3′ to MYO2This study
pRS303myo2tsintegratingHIS3Integrates 3′ to MYO2, replacingThis study
wild-type sequence with myo2ts
pRS306myo2tsintegratingURA3Integrates 3′ to MYO2, replacingThis study
wild-type sequence with myo2ts
YEpSMY12μ circle (high copy number)LEU2SMY1This study
pEG2μ circle (high copy number)URA3, leu2dGST expressed from GAL promoterMitchell et al. 1993
pEGM2μ circle (high copy number)URA3, leu2dGST fused with Myo2p tail,This study
expressed from GAL promoter
pCGcentromere/ARS (low copy number)URA3GST expressed from GAL promoterThis study
pCGMcentromere/ARS (low copy number)URA3GST fused with Myo2p tail,This study
expressed from GAL promoter
pCLcentromere/ARS (low copy number)URA3LexA expressed from GAL promoterThis study
pCLMcentromere/ARS (low copy number)URA3LexA fused with Myo2p tail,This study
expressed from GAL promoter