Table 1

Tight Association between Integrin Translocation and FN Fibril Formation but Not Cell Migration

ConditionsIntegrin translocationFibronectin fibrillogenesisVelocity of cell migration (μm/h)
Control++++36.9 ± 12.0
Pharmacological agents
Cycloheximide(25 μg/ml)5.7 ± 1.8
Cycloheximide/FN(25 μg/ml)++++5.3 ± 1.6
Cytochalasin D(0.4 μM)12.1 ± 3.9
Jasplakinolide(0.1 μM)21.0 ± 5.6
BDM (20 mM)14.7 ± 4.5
Nocodazole(10 μM)++++++9.4 ± 2.7
Vinblastine(50 μM)++++++12.8 ± 3.4
Paclitaxel(20 μM)++++15.5 ± 6.3
PAO (5–50 nM)++++6.5 ± 1.8
Substrates
FN26.1 ± 11.1
FN+VN++++20.7 ± 9.0
FN+PLL10.3 ± 6.5
VN++++47.5 ± 17.6
  • BDM, 2,3-butanedione 2-monoxime; PAO, phenylarsine oxide; and PLL, poly-l-lysine.