Table 1. Summary of GFA1-PP7 mRNP imaging data
REFmex67-5
Particles tracked291203
Nuclear docking events4723
Export events439
Retrograde export events07
Mean export time—dwell time analysis (ms)188aND
Mean export time—MLE (ms)215aND
Mean nuclear docking time during export—dwell time analysis (ms)32a362a
Mean nuclear docking time during export—MLE (ms)39a202a
Mean transition time during export—dwell time analysis (ms)87a406a
Mean transition time during export—MLE (ms)99a383a
Mean cytoplasmic docking time during export—dwell time analysis (ms)62a1,258a
Mean cytoplasmic docking time during export—MLE (ms)77a943a
NE-associated mRNPs per cell0.2 ± 0.4b0.5 ± 0.7b
Nuclear scanning events42
Cytoplasmic scanning events10216
  • a Export data underlying dwell time analysis may not have a normal distribution; consequently, an MLE analysis was performed (Kay, 1993). The differences between dwell times in REF and mex67-5 strains were tested using the distribution independent Wilcoxon rank-sum test and were found to be significant at P < 0.05 for the transition and cytoplasmic docking states. For more details and a discussion of error measurements, see the Materials and methods section Statistical analyses.

  • b Standard deviation.