Table 1

Absence of Incorporation of Mevalonate and HMG-CoA in the Sterol Fraction of T. gondii

Incorporation into the sterol fraction of:
MevalonateHMG-CoA
cpm/mg cell protein
T. gondii130 ± 3588 ± 7
CHO cells455,150 ± 49,8003,670 ± 230
  • Infected or uninfected CHO cells grown for 24 h in medium containing 10% LPDS were incubated with [14C]mevalonate or [14C]HMG-CoA. After washing, the sterol fractions of parasites or CHO cells were isolated to determine the radioactivity content. Values are means ± SD of three separate experiments.