Table 2

Comparison of HMG-CoA Reductase Activity in Untreated or Infected Cells by T. gondii

Infected CHO cellsUninfected CHO cells
pmol mevalonate/min per mg cell protein
3.5% LPDS + LP13.5 ± 2.615.9 ± 4.1
3.5% LPDS29 ± 7.427.1 ± 6.3
  • CHO cells were grown for 24 h in medium containing LP (or not) in the presence or absence of parasites. After washing, cells were permeabilized and incubated in the assay medium containing [14C]HMG-CoA for 30 min at 37°C. The reductase activity was determined by measurement of the production of radioactive mevalonate. Values are means ± SD of three separate experiments.