Table 1

Percentage of Cells Showing Cytoplasmic Staining for Endogenous hnRNP A1, SF2 and hnRNP B1 after Treatment with OSM and UV

3T3 cellsCOS cells
hnRNP A1SF2hnRNP A1SF2hnRNP B1
%%
Control<2<2<2<2<2
0.4 M OSM2 h65–805–1035–4553–5
5 h25–40ND30–35ND2–3
0.6 M OSM2 h>9815–2095–100ND95–100
5 h>98ND90–95ND80–90
UV5 h15–20<23–6<210–15
  • NIH-3T3 and COS cells grown on coverslips were either left untreated or exposed to the indicated stress stimuli, and subsequently fixed and incubated with either the 4B10 antibody to detect endogenous hnRNP A1, the 103 antibody to detect endogenous SF2, or the 2B2 antibody to detect endogenous hnRNP B1.