Table 1

Quantitation of Rab4, Rab5, and Rab11 on Transferrin-labeled Early Endosomes

Rab5Rab4Overlap
Tf endosomes, 30 min internalization51.5 ± 879 ± 423.5 ± 7
Tf endosomes, 2 min internalization, 3 min chase66 ± 668 ± 151 ± 8
Tf endosomes, 30 min internalization, 50 nM wortmannin81 ± 954 ± 447 ± 14
Rab5Rab11Overlap
Tf endosomes, 30 min internalization54 ± 365 ± 119 ± 8
Tf endosomes, 30 min internalization, 50 nM wortmannin88 ± 535 ± 130 ± 7
Rab4Rab11Overlap
Tf endosomes, 30 min internalization84 ± 168 ± 463.5 ± 5
Tf endosomes, 30 min internalization, 50 nM wortmannin55.5 ± 234 ± 635 ± 6
  • Fluorescence analysis: Combinations of two different Rab proteins fused to YFP or CFP were expressed, and Texas red–labeled transferrin was internalized for the indicated time. Numbers represent the percentage of total transferrin-labeled endosomes positive for one or more Rab proteins.