Table 2

Quantitation of Rab4, Rab5, and Rab11 Overlap on the Same Endosomes

Rab5 onlyRab4 and 5Rab5 and 11Rab4, 5, and 11
Rab 5 endosomes48 ± 543 ± 823 ± 215 ± 5
Rab4 onlyRab4 and 5Rab4 and 11Rab4, 5, and 11
Rab 4 endosomes, steady state/2 min + 3 min chase24 ± 4/ND52 ± 6/55 ± 150 ± 1/ND23 ± 1/ND
Rab4 endosomes 50 nM wortmanninND65.5 ± 434.5 ± 9ND
  • Fluorescence analysis: Cells coexpressing YFP-Rab4 and CFP-Rab5 were stained with antibodies against Rab11. Numbers represent the percentage of endosomes labeled for the reference Rab protein only, or combinations of two or three Rab proteins.