Table 2

Saccharomyces cerevisiae Strains Used in This Study

StrainDescriptionReference
SNY36-9AMATahis3-Δ200 leu2-3,112 pho8Δ::ADE2 suc2-Δ9 trp1-901 ura3-52Nothwehr et al. 1995
SNY80SNY36-9A vps35Δ::HIS3This study
SNY110SNY36-9A vps35-101This study
SNY114SNY36-9A vps35-105This study
AHY81SNY36-9A (converted to diploid) vps35-105/vps35-105 vps10/vps10This study
SNY71SNY36-9A pep4Δ::LEU2This study
SHY28SNY36-9A vps10-Q1499LThis study
SHY29SNY36-9A vps10-Q1499Lvps35Δ::HIS3This study
SHY30SNY36-9A vps10-Q1499Lvps35-105This study
SNY132SNY36-9A pep4Δ::LEU2 VPS10::HA vps35Δ::HIS3This study
SNY133SNY36-9A pep4Δ::TRP1 VPS10::HA vps35-105This study
SNY135SNY36-9A pep4Δ::LEU2 vps10-Q1499L::HAThis study
SNY136SNY36-9A pep4Δ::LEU2 vps10-Q1499L::HA vps35Δ::HIS3This study
SNY137SNY36-9A pep4Δ::TRP1 vps10-Q1499L::HA vps35-105This study
W303-1AMATaade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1R. Rothstein
AHY63W303-1A pep4-ΔH3 pho8Δ::ADE2This study
AHY66W303-1A pep4-ΔH3 pho8Δ::ADE2 vps35Δ::HIS3This study
SNY102W303-1A pep4-ΔH3 pho8Δ::ADE2 vps35-101This study
SNY126W303-1A pep4-ΔH3 pho8Δ::ADE2 vps29::HIS3This study
SNY128W303-1A pep4-ΔH3 pho8Δ::ADE2 VPS10::HA vps27Δ::LEU2This study
SNY129W303-1A pep4-ΔH3 pho8Δ::ADE2 VPS10::HA vps27Δ::LEU2 vps35Δ::HIS3This study
SNY134W303-1A pep4-ΔH3 pho8Δ::ADE2 vps10-Q1499L::HA vps27Δ::LEU2 vps35Δ::HIS3This study
SEY6210MATα his3-Δ200 leu2-3,112 lys2-801 suc2-Δ9 trp1-Δ901 ura3-52Robinson et al. 1988
SNY132SEY6210 pep4Δ::TRP1 pho8Δ::LEU2 VPS10::HA vps35Δ::HIS3This study
SNY136SEY6210 pep4Δ::TRP1 pho8Δ::LEU2 vps10-Q1499L::HA vps35Δ::HIS3This study
PBY1SEY6210 pep4Δ::TRP1 pho8Δ::LEU2 vps35Δ::HIS3This study
PBY2SEY6210 pep4Δ::TRP1 pho8Δ::LEU2 vps35-101This study
SHY26SEY6210 pho8-ΔX vps27Δ::LEU2This study
SNY105SEY6210 (converted to diploid) pho8Δ::LEU2/pho8Δ::LEU2 vps35-101/vps35-101This study