Table 1

Effect of MMP-9 on Solubilization of Type II Collagen from Cartilage

AdditiveCollagen solubilized
%
MMP-919 ± 4
MMP-13100
MMP-9 + MMP-13219 ± 7
MMP-9 + MMP-13 + GM60016 ± 2
  • Cartilage discs were incubated for 12 or 24 h in the presence of 50 nM of the indicated MMP. The concentration of MMP inhibitor GM6001 was 10 μM. Collagen solubilizations were normalized to the levels obtained in the presence of MMP-13 (average of 19.7 nmol hydroxyproline per 24 h), and are shown as means ± SD of three independent incubations.