Table 2

Primer Sequences

PrimerSequence
YML067c-NotI5′-ATAAGAATGCGGCCGCAACTCCCCTTCTCCAGACTTG-3′
YML067c-BamHI5′-CGCGGATCCGCGAAGTATGCGGGACGCTG-3′
YAL042w-NotI5′-ATAAGAATGCGGCCGCCTGCAGATGACATTGCGCTGC-3′
YAL042w-BamHI5′-CGCGGATCCGCCATGATCTCTCGGGTTGG-3′
YML067c-pQE-F5′-CGCGGATCCACGAAATGTGATTGGTTGC-3′
YML067c-pQE-R5′-CCCAAGCTTCACCTTTAGCAGCGACATC-3′
YAL042w-pQE-F5′-CGCGGATCCTGTGACCTGGTGAATCTCG-3′
YAL042w-pQE-R5′-CCCAAGCTTCCTGTCCGGGAACACC-3′
OCH1-pQE-F5′-CGCGGATCCGATCCAAGATTTGAAGAAG-3′
OCH1-pQE-R5′-CCCAAGCTTGGGTATGATGAAAGGAGAG-3′
YML067c-KO-F5′-TGCTTTTGCTGTAACGTACAGGCAGAGACTTCAGGAGTATATAGGCCTCCTCTAGTACACTC-3′
YML067c-KO-R5′-GAAACGTTATCTTGGGTATACTGCATTCTCTTTTCTTTTACATATGCGCGCCTCGTTCAGAATG-3′
HIS35′-GCCTCATCCAAAGGCGC-3′
YAL042w-F45′-CGCCATTATAAAGGAAAGCTAGTTTTATGTCTCGTATACAGAATTCGAGCTCGTTTAAAC-3′
YAL042w-R35′-TAGCGAATGCGTCCAGCGACAGCAACGTGGACCTTTTCATGCACTGAGCAGCGTAATCTG-3′
YML067c-F45′-GATATTATCAATGTTCTATTCTTACTGAGAAAAGCGTTCCGAATTCGAGCTCGTTTAAAC-3′
YML067c-R35′-ATAACATACGAAACGCATCAAATGTCTTCAATCCTGCCATGCACTGAGCAGCGTAATCTG-3′
YGR284c-F45′-ACGAAAGATCAAAGGTGTCCTTATTTACTTACAATAGCTGGAATTCGAGCTCGTTTAAAC-3′
YGR284c-R35′-GGCATACCGCCAAAATTTCCAATAGGTCCTCTGTAAGACATGCACTGAGCAGCGTAATCTG-3′
MHF25′-TGGCTTCATTTGGCAAAATGTTCTAATGTGGTTAATGGGTCGGATCCCCGGGTTAATTAA-3′
MHR15′-GCATGAAATTAAATCCTCTCTTTGATCTCTTCAATCAAGAGAATTCGAGCTCGTTTAAAC-3′