Table 1

Measured Parameters of S. pombe Interphase MT Dynamics

MTs, cells
n
MT growth rate Vg
High GFP-tubulin expression level1.86 ± 0.54 μm min−167, 26
Low GFP-tubulin expression level1.90 ± 0.33 μm min−17, 4
Before tip contact2.08 ± 0.53 μm min−117, 10
After tip contact1.30 ± 0.44 μm min−117, 10
Average maximum ratio1.35 ± 0.3331, 23
MT shrinkage rate Vs
High GFP-tubulin expression level8.99 ± 2.82 μm min−164, 26
Low GFP-tubulin expression level9.50 ± 3.85 μm min−17, 4
MT catastrophe frequency fcat0.31 min−149, 19
MT rescue frequency fres0 min−149, 19
Duration of MT cell tip contact before catastrophe1.50 ± 0.59 min179, 33
  • Rates were determined by linear regression analysis of each MT analyzed over 5–20 displacements measured 2–5 s apart. Data are given as mean ± SD of the rates measured for each MT, with n = number of MTs and cells measured.