Table 1

DIAS Analysis of Chemotaxis

StrainSpeedDirection of changeRoundnessDirectionality
μm/mindegree%
Wild-type (KAx-3)9.2 ± 1.6030.2 ± 4.443.3 ± 4.80.74 ± 0.05
pi3k1/2-null4.1 ± 0.8959.3 ± 9.370.2 ± 4.50.29 ± 0.17
PI3K1:pi3k1/2-null9.3 ± 0.9228.4 ± 5.743.2 ± 4.60.73 ± 0.03
phdA-null3.5 ± 0.6061.6 ± 8.654.1 ± 2.90.32 ± 0.08
PhdA/phdA-null8.0 ± 1.5035.6 ± 9.137.3 ± 1.80.70 ± 0.09
PhdA/KAx-38.20 ± 1.6030.1 ± 6.348.9 ± 3.60.73 ± 0.07
PhdAR41C/KAx-35.74 ± 1.0440.4 ± 6.853.3 ± 10.00.59 ± 0.11
Wild-type + LY2940025.0 ± 0.8836.6 ± 4.074.5 ± 1.80.60 ± 0.01
pi3k1/2-null + LY2940024.0 ± 0.3340.4 ± 2.569.3 ± 5.80.57 ± 0.04
PKB overexpressor8.1 ± 1.237.5 ± 7.045.0 ± 5.40.66 ± 0.13
  • DIAS computer analysis was performed from digital time-lapse video differential interference contrast microscopy movies of the listed strains chemotaxing to cAMP. A 40× objective was used for the movies.