Gold particles per membrane length (arbitrary units) ± SD | |||
---|---|---|---|
Nuclear envelope | ER | Nuclear envelope:ER | |
wt-GFP | 0.06 ± 0.07 | 1.31 ± 0.52 | 1:34 |
Δlumen-GFP | 0.29 ± 0.18 | 0.94 ± 0.36 | 1:4 |
GFP-Δcytoplasmic | 0.06 ± 0.06 | 0.54 ± 0.39 | 1:12 |
Δ218–601 | 0.15 ± 0.15 | 0.62 ± 0.39 | 1:9 |
Labeling densities represent the means of 10 cells. Membrane lengths were measured by counting the number of intersections with a squared line lattice of 1 cm^{2} at a magnification of 40,500×. Nuclear envelope/ER ratios were calculated per cell and represent the means of 10 cells.