Table 1

Quantification of Immunogold Labeling of Ultrathin Cryosections of COS Cells Transfected with GFP-tagged CLIMP-63 Constructs

Gold particles per membrane length (arbitrary units) ± SD
Nuclear envelopeERNuclear envelope:ER
wt-GFP0.06 ± 0.071.31 ± 0.521:34
Δlumen-GFP0.29 ± 0.180.94 ± 0.361:4
GFP-Δcytoplasmic0.06 ± 0.060.54 ± 0.391:12
Δ218–6010.15 ± 0.150.62 ± 0.391:9
  • Labeling densities represent the means of 10 cells. Membrane lengths were measured by counting the number of intersections with a squared line lattice of 1 cm2 at a magnification of 40,500×. Nuclear envelope/ER ratios were calculated per cell and represent the means of 10 cells.