Table 3

AUC, CD Spectroscopy Data and Melting Curves

Condition
FragmentsQuantity of ureapH level[Θ]222TmSedimentation coefficient s20, wMolecular mass
M10−3 deg.cm2/dmol °CSkD
127–60118.0−32.64525.72547 (55)
127–6011.58.0−20.3ND80% 26:20% 2ND
127–60128.0−13.7ND50% 26:50% 2ND
127–6012.58.0−5.8ND20% 26:80% 2ND
127–60138.0−4.5ND2.158 (55)
127–60116.0−33.245177.0ND
127–45918.0−31.645>20ND
127–26818.0−14.645>20ND
268–45918.0NDNDND40 (21)
  • Mean molar ellipcities at 222 nm, thermal melting temperatures at the midpoint of transition, sedimentation coefficients, and molecular masses of various CLIMP-63 constructs at different conditions.