Table I.

Summary of protein–protein interactions detected in two-hybrid screens (Continued)

ProteinInteracting protein(s)
Abp1Actin binding protein involved in cortical actin assembly, has SH3 domainCla4a(AD)PAK kinase required for cytokinesis
Rvs167
 (BD)Protein that affects actin distribution and bipolar budding, has an SH3 domain
Srv2
 (AD)Adenylate cyclase–associated protein (CAP) that may provide a link between growth signals and the cytoskeleton
Ynl094wa(AD)Protein of unknown function
Yor284wa(AD)Protein of unknown function
Acf2Protein involved in cortical actin assemblyRvs167a(BD)Protein that affects actin distribution and bipolar budding, has an SH3 domain
Act1Actin, involved in cell polarization, endocytosis, cytoskeletal functionCof1
 (BD)Cofilin, actin binding and severing protein
Las17
 (BD)WASP homologue involved in cortical actin assembly
Pfy1
 (BD)Profilin, can act to prevent actin polymerization and to complex with monomeric actin
Rvs167
 (BD)Protein that affects actin distribution and bipolar budding, has an SH3 domain
Srv2
 (AD,BD)Adenylate cyclase–associated protein (CAP) that may provide a link between growth signals and the cytoskeleton
Aip1Actin interacting protein involved in disassembly of actin filamentsSrv2
 (BD)Adenylate cyclase–associated protein (CAP) that may provide a link between growth signals and the cytoskeleton
Air1RING finger protein that affects RNA processingCdc24a
 (BD)GEF for Cdc42, involved in bud emergence, bud site selection, growth of mating projection
Apg17Protein essential for autophagyApg17a(AD,BD)Protein essential for autophagy
Exo84a(BD)Subunit of the exocyst complex, required for exocytosis
Myo1a(BD)Myosin heavy chain (myosin II), involved in septation and cell wall organization
Nip100a(BD)Mitotic spindle positioning protein, dynactin complex protein associated with the spindle
Rho1–GTPa(BD)GTP-binding protein required to activate the PKC1 pathway and β-1,3-glucan synthase, member of the rho subfamily of ras-like proteins
Rho2–GTPa(BD)GTP-binding protein, member of the rho subfamily of ras-like proteins
Sro77a(BD)Protein that functions together with Sec9p in exocytosis downstream of the Rho3p GTPase
Ykr083ca(AD)Protein of unknown function
Apg7Conjugation protein essential for autophagySso2a(BD)Syntaxin homologue (t-SNARE) involved in vesicle transport from Golgi to plasma membrane
Arp1Actin-related protein of the dynactin complex, required for mitotic spindle orientation and nuclear migrationNip100
 (BD)Mitotic spindle positioning protein, dynactin complex protein associated with the spindle
Bcy1Regulatory subunit of cAMP-dependent protein kinasesSro77a(BD)Protein that functions together with Sec9p in exocytosis downstream of the Rho3p GTPase
Bem1Protein required for cell polarization and bud formation, has two SH3 domainsBoi2
 (BD, AD)Bem1p-binding protein, involved in bud formation,
 has an SH3 domain, Boi1 homologue
Cdc24
 (AD,BD)GEF for Cdc42, involved in bud emergence, bud site selection, growth of mating projection
Cdc42–GDP
 (AD,BD)Rho type GTPase involved in bud site assembly and cell polarity
Cdc42–GTP
 (BD)Rho type GTPase involved in bud site assembly and cell polarity
Bem1
 (continued)Protein required for cell polarization and bud formation, has two SH3 domainsFar1
 (AD)Inhibitor of CDK–cyclin complexes involved in cell cycle arrest for mating and polarized growth of mating projection
Sec15a(AD)Component of exocyst complex required for exocytosis
Ste20
 (AD)PAK kinase of the pheromone pathway; also regulates polarized growth
Swe1a(AD)Serine/tyrosine dual-specificity protein kinase; able to phosphorylate Cdc28p on tyrosine and inhibit its activity
Bem3GAP for Cdc42p and Rho1pBem4a(AD)Bud emergence protein, interacts with Rho type GTPases
Cla4a(AD)PAK kinase required for cytokinesis
Zds1a(AD)Protein with effects on cell polarity and transcriptional silencing, homologue of Zds2
Zds2a(AD)Protein with effects on cell polarity and transcriptional silencing, homologue of Zds1
Bem4Bud emergence protein, interacts with Rho type GTPasesBem3a(BD)GAP for Cdc42p and Rho1p
Cdc11a(AD)Septin, involved in cytokinesis
Cdc12a(AD)Septin, involved in cytokinesis
Cdc24a(BD)GEF for Cdc42, involved in bud emergence, bud site selection, growth of mating projection
Cdc42–GDP
 (BD)Rho type GTPase involved in bud site assembly and cell polarity
Cdc42–GTP
 (BD)Rho type GTPase involved in bud site assembly and cell polarity
Gic1a(BD)Effector of Cdc42p, important for bud emergence, Gic2 homologue
Nfi1a(AD)Septin-interacting protein
Rho1–GTP
 (BD)GTP-binding protein required to activate the PKC1 pathway and β-1,3-glucan synthase, member of the rho subfamily
 of ras-like proteins
Rho2–GTP
 (BD)GTP-binding protein, member of the rho subfamily of ras-like proteins
Rho4–GTP
 (BD)GTP-binding protein of the rho subfamily of ras-like proteins
Rsr1–GDPa(BD)GTP-binding protein of the ras superfamily involved in bud site selection
Rsr1–GTPa(BD)GTP-binding protein of the ras superfamily involved in bud site selection
Spr28a(AD)Septin-related protein expressed during sporulation
Bni1Formin protein involved in cytoskeletal polarization and cytokinesisMsb3a(BD)Protein involved in bud emergence
Pfy1
 (BD)Profilin, can act to prevent actin polymerization and to complex with monomeric actin
Rho1–GTP
 (BD)GTP-binding protein required to activate the PKC1 pathway and β-1,3-glucan synthase, member of the rho subfamily
 of ras-like proteins
Zds2a(BD)Protein with effects on cell polarity and transcriptional silencing, homologue of Zds1
Bni4Protein linking chitin synthase III to septins of
 the neck filamentsMsb2a(BD)Protein involved in bud emergence
Bnr1Bni1p-related formin proteinPfy1
 (BD)Profilin, can act to prevent actin polymerization and to complex with monomeric actin
Boi1Bem1p-binding protein, involved in bud formation, has an SH3 domain, Boi2 homologueSte20
 (AD)PAK kinase of the pheromone pathway; also regulates polarized growth
Yer124ca(AD)Protein of unknown function
Zds2a(AD)Protein with effects on cell polarity and transcriptional silencing, homologue of Zds1
Boi2Bem1p-binding protein, involved in bud formation, has an SH3 domain, Boi1 homologueBem1
 (BD, AD)Protein required for cell polarization and bud formation, has two SH3 domains
Cdc42–GTP
 (BD)Rho type GTPase involved in bud site assembly and cell polarity, GTP bound form
Cla4a(AD)PAK kinase required for cytokinesis
Mrs6a(AD)Rab geranylgeranyltransferase regulatory component (component A) and rab GDI
Msb1a(AD)Protein involved in cell polarity and bud emergence
Ste20
 (AD)PAK kinase of the pheromone pathway; also regulates polarized growth
Yer124ca(AD)Protein of unknown function
Zds2a(AD)Protein with effects on cell polarity and transcriptional silencing, homologue of Zds1
Bub2Protein required for cell cycle arrest in response to loss of microtubule functionGic1a(BD)Effector of Cdc42p, important for bud emergence, Gic2 homologue
Bud2GTPase-activating protein for Rsr1, involved in bud site selectionCln2a(AD)G1/S-specific cyclin
Bud8Protein required for bipolar buddingSte20a(AD)PAK kinase of the pheromone pathway; also regulates polarized growth
Ykl082ca(AD)Protein of unknown function
Cap1Actin-capping protein, α subunitCap2
 (AD)Actin-capping protein, β subunit
Gic2a(AD)Effector of Cdc42p, important for bud emergence, Gic1 homologue
Ypr171wa(AD)Protein of unknown function
Cap2Actin-capping protein, β subunitCap1
 (BD)Actin-capping protein, α subunit
Cdc11Septin, involved in cytokinesisBem4a(BD)Bud emergence protein, interacts with Rho-type GTPases
Cdc12
 (AD,BD)Septin, involved in cytokinesis
Nfi1
 (AD)Septin-interacting protein
Spr28a(AD)Septin-related protein expressed during sporulation
Yor084wa(AD)Protein of unknown function
Zds2a(BD)Protein with effects on cell polarity and transcriptional silencing, homologue of Zds1
Cdc12Septin, involved in cytokinesisBem4a(BD)Bud emergence protein, interacts with Rho-type GTPases
Cdc11
 (AD,BD)Septin, involved in cytokinesis
Cdc12
 (AD,BD)Septin, involved in cytokinesis
Cla4a (BD)PAK kinase required for cytokinesis
Gic1a(BD)Effector of Cdc42p, important for bud emergence, Gic2 homologue
Gic2a(BD)Effector of Cdc42p, important for bud emergence, Gic1 homologue
She3a(AD)Protein required for mother cell-specific expression of HO
Cdc24GEF for Cdc42, involved in bud emergence, bud site selection, growth of mating projectionAir1a(AD)RING finger protein that affects RNA processing
Bem1
 (AD,BD)Protein required for cell polarization and bud formation, has two SH3 domains
Bem4a(AD)Bud emergence protein, interacts with Rho-type GTPases
Cdc24
 (continued)GEF for Cdc42, involved in bud emergence, bud site selection, growth of mating projectionCdc42-GDP
 (BD)Rho-type GTPase involved in bud site assembly and cell polarity
Ent2a(AD)Epsin homologue required for endocytosis
Far1
 (AD)Inhibitor of CDK-cyclin complexes involved in cell cycle arrest for mating and polarized growth of mating projection
Rsr1-GDP
 (BD)GTP-binding protein of the ras superfamily involved in bud site selection
Sec15a(AD)Component of exocyst complex required for exocytosis
Ste20
 (AD)PAK kinase of the pheromone pathway; also regulates polarized growth
Swe1a(AD)Serine/tyrosine dual-specificity protein kinase; able to phosphorylate Cdc28p on tyrosine and inhibit its activity
Ygr221ca(AD)Protein of unknown function
Cdc42–GDPRho type GTPase involved in bud site assembly and cell polarity, GDP-bound formBem1
 (AD)Protein required for cell polarization and bud formation, has two SH3 domains
Bem4
 (AD, BD)Bud emergence protein, interacts with Rho type GTPases
Cdc24
 (AD)GEF for Cdc42, involved in bud emergence, bud site selection, growth of mating projection
Cdc42–GTPRho type GTPase involved in bud site assembly and cell polarity, GTP bound formBem1
 (AD)Protein required for cell polarization and bud formation, has two SH3 domains
Bem4
 (AD)Bud emergence protein, interacts with Rho type GTPases
Boi2
 (AD)Bem1p-binding protein, in-volved in bud formation, has an SH3 domain, Boi1 homologue
Cla4
 (AD)PAK kinase required for cytokinesis
Gic1
 (AD)Effector of Cdc42p, important for bud emergence, Gic2 homologue
Gic2
 (AD)Effector of Cdc42p, important for bud emergence, Gic1 homologue
Rgal
 (AD)Rho-type GTPase activating protein (GAP) for Cdc42
Ste20
 (AD)Serine/threonine protein kinase of the pheromone pathway; also participates in the pathway regulating filamentous growth
Chs4Protein that stimulates chitin synthase III activityYil007ca(AD)Protein of unknown function
Cka1Casein kinase II catalytic (α) subunitRho3–GTPa(BD)GTP-binding protein involved in control of actin cytoskeleton and exocytosis, member of the rho subfamily of ras-like proteins
Cka2Casein kinase II catalytic (α′) subunitRho3–GTPa(BD)GTP-binding protein involved in control of actin cytoskeleton and exocytosis, member of the rho subfamily of ras-like proteins
Ckb1Casein kinase II regulatory (β) subunitRho3–GTPa(BD)GTP-binding protein involved in control of actin cytoskeleton and exocytosis, member of the rho subfamily of ras-like proteins
Cla4PAK kinase required for cytokinesisAbp1a(BD)Actin binding protein involved in cortical actin assembly, has SH3 domain
Bem3a(BD)GAP for Cdc42p and Rho1p
Boi2a(BD)Bem1p-binding protein, involved in bud formation, has an SH3 domain, Boi1 homologue
Cdc12a(AD)Septin, involved in cytokinesis
Cla4
 (continued)PAK kinase required for cytokinesisCdc42–GTP
 (BD)Rho type GTPase involved in bud site assembly and cell polarity
Gic1a(BD)Effector of Cdc42p, important for bud emergence, Gic2 homologue
Gic2a(BD)Effector of Cdc42p, important for bud emergence, Gic1 homologue
Msb2a(BD)Protein involved in bud emergence
Rga1
 (BD)Rho type GTPase-activating protein (GAP) for Cdc42p
Sla2a(BD)Talin-like protein involved in membrane cytoskeleton assembly and required for cell polarization; also required for the internalization phase of endocytosis
Zds2a(BD)Protein with effects on cell polarity and transcriptional silencing, homologue of Zds1
Cln2G1/S-specific cyclinBud2a(BD)GTPase-activating protein for Rsr1, involved in bud site selection
Cof1Cofilin, actin binding and severing proteinAct1
 (AD)Actin, involved in cell polarization, endocytosis, cytoskeletal functions
Crn1Coronin, actin-bundling proteinSvl3a(AD)Protein involved in vacuolar uptake of endocytosed vital dyes
Ynl094wa(AD)Protein of unknown function
Dfg5Protein required for cell polarity, apical growth, and pseudohyphal growthGic1a(BD)Effector of Cdc42p, important for bud emergence, Gic2 homologue
Ent2Epsin homologue required for endocytosisCdc24a(BD)GEF for Cdc42, involved in bud emergence, bud site selection, growth of mating projection
Sro77a(BD)Protein that functions together with Sec9p in exocytosis downstream of the Rho3p GTPase
Exo84Subunit of the exocyst complex, required for exocytosisApg17a(AD)Protein essential for autophagy response to nutritional stress
Sec15
 (AD)Component of exocyst complex required for exocytosis
Far1Inhibitor of CDK-cyclin complexes involved in cell cycle arrest for mating and polarized growth of mating projectionBem1
 (BD)Protein required for cell polarization and bud formation, has two SH3 domains
Cdc24
 (BD)GEF for Cdc42, involved in bud emergence, bud site selection, growth of mating projection
Gic1Effector of Cdc42p, important for bud emergence, Gic2 homologueBem4a(AD)Bud emergence protein, interacts with Rho-type GTPases
Bub2a(AD)Protein required for cell cycle arrest in response to loss of microtubule function
Cdc12a(AD)Septin, involved in cytokinesis
Cdc42-GTP
 (BD)Rho-type GTPase involved in bud site assembly and cell polarity
Cla4a(AD)PAK kinase required for cytokinesis
Dfg5a(AD)Protein required for cell polarity, apical growth, and pseudohyphal growth
Gic1
 (AD,BD)Effector of Cdc42p, important for bud emergence, Gic2 homologue
Gic2
 (AD,BD)Effector of Cdc42p, important for bud emergence, Gic1 homologue
Hof1a(AD)Protein involved in cytokinesis, has an SH3 domain
Ste50a(AD)Protein required for feedback control of pheromone-induced signal transduction
Ykl082ca(AD)Protein of unknown function
Ycr086wa(AD)Protein of unknown function
Gic1
 (continued)Effector of Cdc42p, important for bud emergence, Gic2 homologueZds1a(AD)Protein with effects on cell polarity and transcriptional silencing, homologue of Zds2
Zds2a(AD)Protein with effects on cell polarity and transcriptional silencing, homologue of Zds1
Gic2Effector of Cdc42p, important for bud emergence, Gic1 homologueCap1a(AD)Actin-capping protein, α subunit
Cdc12a(AD)Septin, involved in cytokinesis
Cdc42-GTP
 (BD)Rho-type GTPase involved in bud site assembly and cell polarity
Cla4a(AD)PAK kinase required for cytokinesis
Gic1
 (AD,BD)Effector of Cdc42p, important for bud emergence, Gic2 homologue
Rga1a(BD)Rho-type GTPase-activating protein (GAP) for Cdc42p
Ste50a(AD)Protein required for feedback control of pheromone-induced signal transduction
Ycr086wa(AD)Protein of unknown function
Ykl082ca(AD)Protein of unknown function
Zds1a(AD)Protein with effects on cell polarity and transcriptional silencing, homologue of Zds2
Zds2a(AD)Protein with effects on cell polarity and transcriptional silencing, homologue of Zds1
Hof1Protein involved in cytokinesis, has an SH3 domainGic1a(BD)Effector of Cdc42p, important for bud emergence, Gic2 homologue
Hsl7Negative regulatory protein of the Swe1p protein kinaseYnl094w
 (AD)Protein of unknown function
Kin3Serine/threonine protein kinase, unknown functionSro77a(BD)Protein that functions together with Sec9p in exocytosis downstream of the Rho3p GTPase
Las17WASP homologue involved in cortical actin assemblyAct1
 (AD)Actin, involved in cell polarization, endocytosis, cytoskeletal functions
Rvs167
 (AD, BD)Protein that affects actin distribution and bipolar budding, has an SH3 domain
Sla1
 (BD)Protein involved in assembly of cortical actin cytoskeleton, has three SH3 domains
Yhr133ca(AD)Protein of unknown function
Ypl246ca(AD)Protein of unknown function
Mrs6Rab geranylgeranyltransferase regulatory subunit and rab GDIBoi2a(BD)Bem1p-binding protein, involved in bud formation, has an SH3 domain, Boi1 homologue
Msb1Protein involved in cell polarity and bud emergenceBoi2a(BD)Bem1p-binding protein, involved in bud formation, has an SH3 domain, Boi1 homologue
Msb2Protein involved in bud emergenceBni4a(AD)Protein linking chitin synthase III to septins of the neck filaments
Cla4a(AD)PAK kinase required for cytokinesis
Msb3Protein involved in bud emergenceBni1a(AD)Formin protein involved in cytoskeletal polarization and cytokinesis
Spa2a(AD)Protein involved in cell polarity and cell fusion during mating
Msb4Protein involved in bud emergenceSpa2a(AD)Protein involved in cell polarity and cell fusion during mating
Myo1Myosin heavy chain (myosin II), involved in septation and cell wall organizationApg17a(AD)Protein essential for autophagy response to nutritional stress
Spr3a(AD)Sporulation-specific septin
Ypr188ca(AD)Protein of unknown function
Nfi1Septin-interacting proteinBem4a(BD)Bud emergence protein, interacts with Rho type GTPases
Cdc11a(BD)Septin, involved in cytokinesis
Nip100a(BD)Mitotic spindle positioning protein, dynactin complex protein associated with the spindle
Nip100Mitotic spindle positioning protein, dynactin complex protein associated with the spindleApg17a(AD)Protein essential for autophagy response to nutritional stress
Arp1
 (AD)Actin-related protein of the dynactin complex, required for mitotic spindle orientation and nuclear migration
Nfi1a(AD)Septin-interacting protein
Nip100
 (AD,BD)Mitotic spindle positioning protein, dynactin complex protein associated with the spindle
Pac11
 (AD)Protein with similarity to rat dynein intermediate chain; required in the absence of Cin8p, member of WD (WD-40) repeat family
Rho1–GTPa(BD)GTP-binding protein required to activate the PKC1 pathway and β-1,3-glucan synthase, member of the rho subfamily of ras-like proteins
Rho2–GTPa(BD)GTP-binding protein, member of the rho subfamily of ras-like proteins
Sro77a(BD)Protein that functions together with Sec9p in exocytosis downstream of the Rho3p GTPase
Pac11Protein with similarity to rat dynein intermediate chainNip100
 (BD)Mitotic spindle positioning protein, dynactin complex protein associated with the spindle
Pfs1Protein required for sporulationYil007ca(BD)Protein of unknown function
Pfy1Profilin, can act to prevent actin polymerization and to complex with monomeric actinAct1
 (AD)Actin, involved in cell polarization, endocytosis, cytoskeletal functions
Bni1
 (AD)Formin protein involved in cytoskeletal polarization and cytokinesis
Bnr1
 (AD)Bni1p-related formin protein
Srv2
 (AD)Adenylate cyclase–associated protein (CAP) that may provide a link between growth signals and the cytoskeleton
Pkc1Protein kinase C, regulates MAP kinase cascade involved in regulating cell wall metabolismRho1–GTP(BD)GTP-binding protein required to activate the PKC1 pathway and β-1,3-glucan synthase, member of the rho subfamily of ras-like proteins
Ygr221Ca(BD)Protein of unknown function
Zds2a(BD)Protein with effects on cell polarity and transcriptional silencing, homologue of Zds1
Rga1Rho type GTPase-activating protein (GAP) for Cdc42pCdc42
 (BD)Rho-type GTPase involved in bud site assembly and cell polarity
Cla4a(AD)PAK kinase required for cytokinesis
Gic2a
 (AD)Effector of Cdc42p, important for bud emergence, Gic1 homologue
Rho1
 (AD,BD)GTP-binding protein required to activate the PKC1 pathway and β-1,3-glucan synthase, member of the rho subfamily of ras-like proteins
Rho1–GTPGTP-binding protein required to activate the PKC1 pathway and β-1,3-glucan synthase, member of the rho subfamily of ras-like proteins, GTP-bound formRga1
 (AD,BD)Rho type GTPase-activating protein (GAP) for Cdc42 and Rho1
Apg17a
 (AD)Protein essential for autophagy response to nutritional stress
Bem4
 (AD)Bud emergence protein, interacts with Rho type GTPases
Bni1
 (AD)Formin protein involved in cytoskeletal polarization and cytokinesis
Rho1–GTP
 (continued)GTP-binding protein required to activate the PKC1 pathway and β-1,3-glucan synthase, member of the rho subfamily of ras-like proteins, GTP-bound formNip100a
 (AD)Mitotic spindle positioning protein, dynactin complex protein associated with the spindle
Pkc1
 (AD)Protein kinase C, regulates MAP kinase cascade involved in regulating cell wall metabolism
Shc1a
 (AD)Protein involved in cell wall chitin synthesis or deposition
Yil007ca
 (AD)Protein of unknown function
Zds2a
 (AD)Protein with effects on cell polarity and transcriptional silencing, homologue of Zds1
Rho2–GTPGTP-binding protein, member of the rho subfamily of ras-like proteins, GTP bound formApg17a
 (AD)Protein essential for autophagy response to nutritional stress
Bem4
 (AD)Bud emergence protein, interacts with Rho type GTPases
Nip100a
 (AD)Mitotic spindle positioning protein, dynactin complex protein associated with the spindle
Rho3–GTPGTP-binding protein, member of the rho subfamily of ras-like proteins, GTP bound formCka1a
 (AD)Casein kinase II catalytic (α) subunit
Cka2a
 (AD)Casein kinase II catalytic (α′) subunit
Ckb1a
 (AD)Casein kinase II regulatory (β) subunit
Rho4–GTPGTP-binding protein of the rho subfamily of ras-like proteins, GTP bound formBem4
 (AD)Bud emergence protein, interacts with Rho type GTPases
Yil007ca
 (AD)Protein of unknown function
Rpn4Subunit of the regulatory particle of the proteasomeYil007ca
 (BD)Protein of unknown function
Rsr1-GDPGTP-binding protein of the ras superfamily involved in bud site selection, GDP-bound formBem4a
 (AD)Bud emergence protein, interacts with Rho type GTPases
Cdc24
 (AD)GEF for Cdc42, involved in bud emergence, bud site selection, growth of mating projection
Sec15a
 (AD)Component of exocyst complex required for exocytosis
Rsr1–GTPGTP-binding protein of the ras superfamily involved in bud site selection, GTP-bound formBem4a
 (AD)Bud emergence protein, interacts with Rho type GTPases
Rvs161Protein required for viability after N, C, or S starvation, for internalization step of endocytosis, and for cell fusion during mating; roles in endocytosis and in cell fusion are independent of one anotherRvs167
 (AD,BD)Protein that affects actin distribution and bipolar budding, has an SH3 domain
Rvs167Protein that affects actin distribution and bipolar budding, has an SH3 domainAbp1
 (AD)Actin binding protein involved in cortical actin assembly, has SH3 domain
Acf2a
 (AD)Protein involved in cortical actin assembly
Act1
 (AD)Actin, involved in cell polarization, endocytosis, cytoskeletal functions
Las17
 (AD, BD)WASP homologue involved in cortical actin assembly
Rvs161
 (AD, BD)Protein required for viability after N, C, or S starvation, for internalization step of endocytosis, and for cell fusion during mating; roles in endocytosis and in cell fusion are independent of one another
Rvs167
 (AD, BD)Protein that affects actin distribution and bipolar budding, has an SH3 domain
Sla1
 (BD)Protein involved in assembly of cortical actin cytoskeleton, has three SH3 domains
Sla2
 (AD)Talin-like protein involved in membrane cytoskeleton assembly and required for cell polarization; also required for the internalization phase of endocytosis
Srv2
 (AD)Adenylate cyclase–associated protein (CAP) that may provide a link between growth signals and the cytoskeleton
Rvs167
 (continued)Protein that affects actin distribution and bipolar budding, has an SH3 domainYbr108c
 (AD)Protein of unknown function
Yjr083ca
 (AD)Protein of unknown function
Ygr268ca
 (AD)Protein of unknown function
Ynl086wa
 (AD)Protein of unknown function
Ynl094wa
 (AD, BD)Protein of unknown function
Yor284wa
 (AD)Protein of unknown function
Ypr171wa
 (AD)Protein of unknown function
Ysc84a
 (AD)Protein of unknown function, has an SH3 domain
Sec15Component of exocyst complex required for exocytosisBem1a
 (BD)Protein required for cell polarization and bud formation, has two SH3 domains
Cdc24a
 (BD)GEF for Cdc42, involved in bud emergence, bud site selection, growth of mating projection
Exo84
 (BD)Subunit of the exocyst complex, required for exocytosis
Rsr1–GDPa
 (BD)GTP-binding protein of the ras superfamily involved in bud site selection
Shc1Protein involved in cell wall chitin synthesis or depositionRho1–GTPa
 (BD)GTP-binding protein required to activate the PKC1 pathway and β-1,3-glucan synthase, member of the rho subfamily of ras-like proteins
She3Protein required for mother cell-specific expression of HOCdc12a
 (BD)Septin, involved in cytokinesis
Sla1Protein involved in assembly of cortical actin cytoskeleton, has three SH3 domainsLas17
 (AD)WASP homologue involved in cortical actin assembly
Rvs167
 (AD)Protein that affects actin distribution and bipolar budding, has an SH3 domain
Srv2
 (AD)Adenylate cyclase–associated protein (CAP) that may provide a link between growth signals and the cytoskeleton
Ygr268ca
 (AD)Protein of unknown function
Ynl094wa
 (AD)Protein of unknown function
Ypr171wa
 (AD)Protein of unknown function
Ysc84a
 (AD)Protein of unknown function, has an SH3 domain
Sla2Talin-like protein involved in membrane cytoskeleton assembly and required for cell polarization; also required for the internalization phase of endocytosisCla4a
 (AD)PAK kinase required for cytokinesis
Rvs167
 (BD)Protein that affects actin distribution and bipolar budding, has an SH3 domain
Ynl094wa
 (AD)Protein of unknown function
Yor284wa
 (AD)Protein of unknown function
Ysc84a
 (AD)Protein of unknown function, has an SH3 domain
Spa2Protein involved in cell polarity and cell fusion during matingMsb3a
 (BD)Protein involved in bud emergence
Msb4a
 (BD)Protein involved in bud emergence
Spr28Septin-related protein expressed during sporulationBem4a
 (BD)Bud emergence protein, interacts with Rho type GTPases
Cdc11a
 (BD)Septin, involved in cytokinesis
Spr3Sporulation-specific septinMyo1a
 (BD)Myosin heavy chain (myosin II), involved in septation and cell wall organization
Spr6Sporulation-specific proteinZds2a
 (BD)Protein with effects on cell polarity and transcriptional silencing, homologue of Zds1
Sro77Protein that functions together with Sec9p in exocytosis downstream of the Rho3p GTPaseApg17a
 (AD)Protein essential for autophagy response to nutritional stress
Bcy1a
 (AD)Regulatory subunit of cAMP-dependent protein kinases
Ent2a
 (AD)Epsin homologue required for endocytosis
Kin3a
 (AD)Serine/threonine protein kinase, unknown function
Sro77
 (continued)Protein that functions together with Sec9p in exocytosis downstream of the Rho3p GTPaseNip100a
 (AD)Mitotic spindle positioning protein, dynactin complex protein associated with the spindle
Yap1801a
 (AD)Protein homologous to clathrin assembly polypeptide AP180; interacts with Pan1p
Yip1a
 (AD)Protein involved in vesicular transport; interacts with transport GTPases Ypt1p and Ypt31p at the Golgi membrane
Ynl094wa
 (AD)Protein of unknown function
Yor197wa
 (AD)Protein of unknown function
Srv2Adenylate cyclase-associated protein (CAP) that may provide a link between growth signals and the cytoskeletonAbp1(BD)Actin binding protein involved in cortical actin assembly, has SH3 domain
Act1
 (AD,BD)Actin, involved in cell polarization, endocytosis, cytoskeletal functions
Aip1
 (AD)Actin interacting protein involved in disassembly of actin filaments
Pfy1
 (BD)Profilin, can act to prevent actin polymerization and to complex with monomeric actin
Rvs167
 (BD)Protein that affects actin distribution and bipolar budding, has an SH3 domain
Sla1
 (BD)Protein involved in assembly of cortical actin cytoskeleton, has three SH3 domains
Srv2
 (AD,BD)Adenylate cyclase-associated protein (CAP) that may provide a link between growth signals and the cytoskeleton
Yhr070wa(AD)Protein of unknown function
Sso1Syntaxin homologue (t-SNARE) involved in vesicle transport from Golgi to plasma membraneYap1801
 (AD)Protein homologous to clathrin assembly polypeptide AP180; interacts with Pan1p
Sso2Syntaxin homologue (t-SNARE) involved in vesicle transport from Golgi to plasma membraneApg7a(AD)Protein essential for autophagy response to nutritional stress
Yap1801
 (AD)Protein homologous to clathrin assembly polypeptide AP180; interacts with Pan1p
Ste20Serine/threonine protein kinase of the pheromone pathway; also participates in the pathway regulating filamentous growthBem1
 (AD)Protein required for cell polarization and bud formation, has two SH3 domains
Bem4a(AD)Bud emergence protein, interacts with Rho-type GTPases
Boi1
 (AD)Bem1p-binding protein, involved in bud formation, has an SH3 domain, Boi2 homologue
Boi2
 (AD)Bem1p-binding protein, involved in bud formation, has an SH3 domain, Boi1 homologue
Bud8a(BD)Protein required for bipolar budding
Cdc42-GTP
 (BD)Rho-type GTPase involved in bud site assembly and cell polarity
Ste50Protein required for feedback control of pheromone-induced signal transductionGic1
 (BD)Effector of Cdc42p, important for bud emergence, Gic2 homologue
Gic2
 (BD)Effector of Cdc42p, important for bud emergence, Gic1 homologue
Svl3Protein involved in vacuolar uptake of endocytosed
 vital dyesCrn1a(BD)Coronin, actin-bundling protein
Swe1Serine/tyrosine dual-specificity protein kinase; able to phosphorylate Cdc28p on tyrosine and inhibit its activityBem1a(BD)Protein required for cell polarization and bud formation, has two SH3 domains
Swe1
 (continued)Serine/tyrosine dual-specificity protein kinase; able to phosphorylate Cdc28p on tyrosine and inhibit its activityCdc24a(BD)GEF for Cdc42, involved in bud emergence, bud site selection, growth of mating projection
Ynl094wa(AD)Protein of unknown function
Yal004wProtein of unknown functionZds2a(BD)Protein with effects on cell polarity and transcriptional silencing, homologue of Zds1
Yap1801Protein homologous to clathrin assembly polypeptide AP180; interacts with Pan1pSro77a(BD)Protein that functions together with Sec9p in exocytosis downstream of the Rho3p GTPase
Sso1
 (BD)Syntaxin homologue (t-SNARE) involved in vesicle transport from Golgi to plasma membrane
Sso2
 (BD)Syntaxin homologue (t-SNARE) involved in vesicle transport from Golgi to plasma membrane
Ybr108cProtein of unknown functionRvs167a(BD)Protein that affects actin distribution and bipolar budding, has an SH3 domain
Ycr086wProtein of unknown functionGic1a(BD)Effector of Cdc42p, important for bud emergence, Gic2 homologue
Gic2a(BD)Effector of Cdc42p, important for bud emergence, Gic1 homologue
Yel023Protein of unknown functionZds2a(BD)Protein with effects on cell polarity and transcriptional silencing, homologue of Zds1
Yer124cProtein of unknown functionBoi1a(BD)Bem1p-binding protein, involved in bud formation, has an SH3 domain, Boi1 homologue
Boi2a(BD)Bem1p-binding protein, in-volved in bud formation, has an SH3 domain, Boi1 homologue
Zds2a(BD)Protein with effects on cell polarity and transcriptional silencing, homologue of Zds1
Ygr221cProtein of unknown functionCdc24a (BD)GEF for Cdc42, involved in bud emergence, bud site selection, growth of mating projection
Pkc1a(AD)Protein kinase C; regulates MAP kinase cascade involved in regulating cell wall metabolism
Ygr268cProtein of unknown functionRvs167a
 (BD)Protein that affects actin distribution and bipolar budding, has an SH3 domain
Sla1a
 (BD)Protein involved in assembly of cortical actin cytoskeleton, has three SH3 domains
Yhr070wProtein of unknown functionSrv2a
 (BD)Adenylate cyclase-associated protein (CAP) that may provide a link between growth signals and the cytoskeleton
Yhr133cProtein of unknown functionLas17a
 (BD)WASP homologue involved in cortical actin assembly
Yhr149CProtein of unknown functionZds1a
 (AD)Protein with effects on cell polarity and transcriptional silencing, homologue of Zds2
Zds2a
 (AD)Protein with effects on cell polarity and transcriptional silencing, homologue of Zds1
Yil007CProtein of unknown functionChs4a
 (AD)Protein that stimulates chitin synthase III activity
Pfs1a
 (AD)Protein required for sporulation
Rho1–GTPa
 (BD)GTP-binding protein required to activate the PKC1 pathway and β-1,3-glucan synthase, member of the rho subfamily of ras-like proteins
Rho4–GTPa
 (BD)GTP-binding protein of the rho subfamily of ras-like proteins
Rpn4a
 (AD)Subunit of the regulatory particle of the proteasome
Yip1Protein involved in vesicular transport; interacts with transport GTPases Ypt1p and Ypt31p at the Golgi membraneSro77a
 (BD)Protein that functions together with Sec9p in exocytosis downstream of the Rho3p GTPase
Yjr083cProtein of unknown functionRvs167a
 (BD)Protein that affects actin distribution and bipolar budding, has an SH3 domain
Ykl082cProtein of unknown functionBud8a
 (BD)Protein required for bipolar budding
Gic1a
 (BD)Effector of Cdc42p, important for bud emergence, Gic2 homologue
Gic2a
 (BD)Effector of Cdc42p, important for bud emergence, Gic1 homologue
Zds2a
 (BD)Protein with effects on cell polarity and transcriptional silencing, homologue of Zds1
Ykr083cProtein of unknown functionApg17a
 (BD)Protein essential for autophagy response to nutritional stress
Ynl086wProtein of unknown functionRvs167a
 (BD)Protein that affects actin distribution and bipolar budding, has an SH3 domain
Ynl094wProtein of unknown functionAbp1a
 (BD)Actin binding protein involved in cortical actin assembly, has SH3 domain
Crn1a
 (BD)Coronin, actin-bundling protein
Hsl7
 (BD)Negative regulatory protein of the Swe1p protein kinase
Rvs167a
 (AD,BD)Protein that affects actin distribution and bipolar budding, has an SH3 domain
Sla1a
 (BD)Protein involved in assembly of cortical actin cytoskeleton, has three SH3 domains
Sla2a
 (BD)Talin-like protein involved in membrane cytoskeleton assembly and required for cell polarization; also required for the internalization phase of endocytosis
Sro77a
 (BD)Protein that functions together with Sec9p in exocytosis downstream of the Rho3p GTPase
Swe1a
 (BD)Serine/tyrosine dual-specificity protein kinase; able to phosphorylate Cdc28p on tyrosine and inhibit its activity
Yor084wProtein of unknown functionCdc11a
 (BD)Septin, involved in cytokinesis
Yor197wProtein of unknown functionSro77a
 (BD)Protein that functions together with Sec9p in exocytosis downstream of the Rho3p GTPase
Yor284wProtein of unknown functionAbp1a
 (BD)Actin binding protein involved in cortical actin assembly, has SH3 domain
Rvs167a
 (BD)Protein that affects actin distribution and bipolar budding, has an SH3 domain
Sla2a
 (BD)Talin-like protein involved in membrane cytoskeleton assembly and required for cell polarization; also required for the internalization phase of endocytosis
Ypl246cProtein of unknown functionLas17a
 (BD)WASP homologue involved in cortical actin assembly
Ypr171wProtein of unknown functionCap1a
 (BD)Actin-capping protein, α subunit
Rvs167a
 (BD)Protein that affects actin distribution and bipolar budding, has an SH3 domain
Sla1a
 (BD)Protein involved in assembly of cortical actin cytoskeleton, has three SH3 domains
Ypr188cProtein of unknown functionMyo1a
 (BD)Myosin heavy chain (myosin II), involved in septation and cell wall organization
Ysc84Protein of unknown function, has an SH3 domainRvs167a(BD)Protein that affects actin distribution and bipolar budding, has an SH3 domain
Sla1a(BD)Protein involved in assembly of cortical actin cytoskeleton, has three SH3 domains
Sla2a(BD)Talin-like protein involved in membrane cytoskeleton assembly and required for cell polarization; also required for the internalization phase of endocytosis
Zds1Bem3a(BD)GAP for Cdc42p and Rho1p
Gic1a(BD)Effector of Cdc42p, important for bud emergence, Gic2 homologue
Gic2a(BD)Effector of Cdc42p, important for bud emergence, Gic1 homologue
Yhr149ca(BD)Protein of unknown function
Zds2a(AD,BD)Protein with effects on cell polarity and transcriptional silencing, homologue of Zds1
Zds2Protein with effects on cell polarity and transcriptional silencing, homologue of Zds1Bem3a(BD)GAP for Cdc42p and Rho1p
Bni1a(AD)Formin protein involved in cytoskeletal polarization and cytokinesis
Boi1a(BD)Bem1p-binding protein, in-volved in bud formation, has an SH3 domain, Boi2 homologue
Boi2a(BD)Bem1p-binding protein, in-volved in bud formation, has an SH3 domain, Boi1 homologue
Cdc11a(AD)Septin, involved in cytokinesis
Cla4a(AD)PAK kinase required for cytokinesis
Gic1a(BD)Effector of Cdc42p, important for bud emergence, Gic2 homologue
Gic2a(BD)Effector of Cdc42p, important for bud emergence, Gic1 homologue
Pkc1a(AD)Protein kinase C; regulates MAP kinase cascade involved in regulating cell wall metabolism
Rho1-GTPa(BD)GTP-binding protein required to activate the PKC1 pathway and beta-1,3-glucan synthase, member of the rho subfamily of ras-like proteins
Spr6aa(AD)Sporulation-specific protein
Yal004wa(AD)Protein of unknown function
Yel023ca(AD)Protein of unknown function
Yer124ca(AD)Protein of unknown function
Yhr149ca(BD)Protein of unknown function
Ykl082ca(AD)Protein of unknown function
Zds1a(AD,BD)Protein with effects on cell polarity and transcriptional silencing, homologue of Zds2
Zds2a(AD,BD)Protein with effects on cell polarity and transcriptional silencing, homologue of Zds1
  • a Interactions not previously identified.

  • A total of 191 reproducible two-hybrid interactions involving 110 proteins were detected. Proteins are listed in alphabetical order. Each pairwise interaction appears twice in the table, once under the bait protein and once under the interacting prey protein. Entries in the second column are noted as BD (binding domain) or AD (activation domain) to signify the direction of the two-hybrid interaction.