Table II.

Cellular localization of YFP-tagged proteins encoded by novel ORFs

ProteinNull mutant
 phenotypeTwo-hybrid
 interactionsHomologuesaYFP fusion
 locationFigure
Ykl082cLethalbBud8, Zds2, Gic1,
 Gic2D. melanogaster CG13648 (21%); CG9274 (22%)
 C. elegans ZK354.3 (25%); C17F3.3 (27%)
 S. pombe Spac8c9.10cp (24%); Spbc1861.01cp (20%)NucleolusFig. 4, A and B
Ycr086wViable, benomyl
 sensitivecGic1, Gic2None knownPunctate localization
 at nuclear periphery,
 nuclear envelopeFig. 4, C and D
Ygr221cViable, sensitive to
 nonhydrolyzable
 GDP analoguescCdc24, Pkc1S. cerevisiae Yhr149c (32%); Muc1(23%)Bud tip, bud neckFig. 4, E and F
Yhr149cViablebZds1, Zds2S. cerevisiae Ygr221c (32%)Bud tip, bud neckPFig. 4, O and P
Yil079c/Air1ViablebCdc24H. sapiens ZNF9 (26%)
 M. musculus CNBP (26%)
 D. melanogaster CG9715 (28%); CG3800 (28%)
 C. elegans GLH-4 (25%)NucleolusFig. 4, G and H
Yer124cViablebBoi1, Boi2, Zds2D. melanogaster DS02740.2:BG:DS02740.2 (32%)No detectable signalData not shown
Yil007cViablebRho1, Rho4, Pfs1,
 Chs4, Rpn4H. sapiens PSMD9 (35%)
 D. melanogaster CG9588 (28%)
 C. elegans C44B7.1 (31%); Y42H9AR.F (30%)
 S. pombe Spac2h10.02cp (27%)CytoplasmData not shown
Ylr423c/Apg17ViablebRho1, Rho2, Apg17,
 Exo84, Myo1, Nip100,
 Sro77, Ykr083cS. cerevisiae Ynl047p (24%)Punctate localization
 in cytoplasmFig. 4, I and J
Ypr171wViablebCap1, Rvs167, Sla1H. sapiens USP8 (23%)
 D. melanogaster CG13648 (22%)
 S. cerevisiae Crp1 (25%)Actin patchesFigs. 4, K and L,
 and 5
Ygr268cViablebRvs167, Sla1S. pombe Spac17a5.10p (31%)CytoplasmData not shown
Yor284wViablebAbp1, Rvs167, Sla2None known2–5 fast moving dots
 around cell peripheryFigs. 4, M and N,
 and 6
Yjr083cViablebRvs167None knownNo detectable signalData not shown
Ynl094wViablebAbp1, Crn1, Hsl7,
 Rvs167, Sla1,
 Sla2, Sro77, Swe1S. pombe Spbc29b5.04cp (26%)Actin patchesUnpublished
 data
  • a Information on homologues from other species is taken from the Yeast Proteome Database at http://www.proteome.com.

  • b Information on these phenotypes of null mutants is taken from Winzeler et al. (1999).

  • c Information on these phenotypes of null mutants is taken from Rieger et al. (1999).

  • Proteins are listed in the order in which they are discussed in the text. Percentages refer to amino acid identity between homologues.