Table I.

Semiquantitative analysis of Purkinje cell engraftment

MouseGFP labellingTime
 post-BMTGFP+ microgliaGFP+ cells
 Perivascular/leptomeningealGFP+Purkinje cellsTotal
 Purkinje cells
mo
1Retroviral121,1072,5151013,519
2Retroviral122,0642,9891013,691
3Transgenic107471,331213,110
  • 10–12 mo after transplantation of BM cells expressing GFP under the control of a retroviral or β-actin promoter, every seventh cerebellar section of the chimeric mice was analyzed for GFP-expressing cells. Purkinje cells and microglia were identified by their morphology and immunoreactivity for calbindin/GAD and Iba1, respectively. GFP-positive cells located in perivascular and leptomeningeal sites were also evaluated.