Table I.

Strains used in this study

StrainGenotypea
LHY1his4 leu2 lys2 ura3 bar1-1
LHY10ste2Δ::LEU2 his3 leu2 trp1 ura3 bar1-1
LHY37end4::LEU2 his4 leu2 lys2 ura3 bar1-1
LHY291his3 leu2 lys2 trp1 ura3 bar1-1
LHY558ste2Δ::LEU2 his3 leu2 trp1 ura3::STE24XR-UbK48R::URA3 bar1-1
LHY825ste2Δ::LEU2 his3 leu2 trp1 ura3::STE2378Stop::URA3 bar1-1
LHY1451ste2Δ::TRP1 GAL2 ade2 his3 leu2 lys2 trp1 ura3 bar1-1 pGalSTE2-378Stop[URA3]
LHY2536ypk1Δ::KAN his3 leu2Δ met15Δ ura3 bar1-1
LHY2537his3 leu2 trp1 ura3
LHY2543ypk1G490RGAL2 his3 leu2 lys2 trp1 ura3 bar1-1
LHY2563ypk1Δ::KAN his3::YPK1::HIS3 leu2Δ met15Δ ura3 bar1-1
LHY2564ypk1Δ::KAN his3::YPK1K376R::HIS3 leu2Δ met15Δ ura3 bar1-1
LHY2565ypk1Δ::KAN his3::YPK1D488A::HIS3 leu2Δ met15Δ ura3 bar1-1
LHY2567ypk1Δ::KAN his3::YPK1T662A::HIS3 leu2Δ met15Δ ura3 bar1-1
LHY2568ypk1Δ::KAN his3::YPK1T504A::HIS3 leu2Δ met15Δ ura3 bar1-1
LHY2569ypk1Δ::KAN his3::YPK1T504A,T662A::HIS3 leu2Δ met15Δ ura3 bar1-1
LHY2632his3Δ leu2Δ met15Δ ura3Δ bar1Δ::URA3
LHY2633ypk2Δ::KAN his3Δ leu2Δ met15Δ ura3Δ bar1Δ::URA3
LHY2684ypk1G490Rste2Δ::LEU2 GAL2 his3 leu2 lys2 trp1 ura3::STE2378Stop::URA3 bar1-1
LHY2690ypk1G490Rste2Δ::LEU2 GAL2 his3 leu2 lys2 trp1 ura3::STE24XR-UbK48R::URA3 bar1-1
LHY2712ypk1G490RGAL2 his3 leu2 lys2 trp1 ura3 bar1 pYPK1[URA3]
LHY2714pkh1Δ::KAN pkh2Δ::KAN his3Δ leu2::PKH1::LEU2 met15Δ ura3Δ bar1-1
LHY2716pkh1Δ::KAN pkh2Δ::KAN his3Δ leu2 met15Δ ura3Δ bar1-1
LHY2759pkh1Δ::KAN his3 leu2 trp1 ura3 bar1-1
LHY2760pkh2Δ::KAN his3 leu2 trp1 ura3 bar1-1
LHY2761MATα GAL2 his3 leu2 lys2 trp1 ura3 bar1-1
LHY2762MATα leu2 his3 met15 ura3
LHY3030pkh1D398Gpkh2Δ::LEU2 ade1 his2 leu2 trp1 ura3 bar1Δ::URA3
LHY3031pkh1Δ::LEU2 ade1 his2 leu2 trp1 ura3 bar1Δ::URA3
LHY3032pkh2Δ::LEU2 ade1 his2 leu2 trp1 ura3 bar1Δ::URA3
  • a All strains are MATa unless otherwise noted.