Table I.

Two-hybrid interaction between Ypt32p and Sec2p

Ypt32 constructAmino acid changeClassSec2pa
Wild type+
S27Ser27→AsnBinds GDP preferentially
E49Glu49→GlnEffector domain?+
Q72Gln72→LeuGTPase deficient+
N126Asn126→IleNucleotide binding deficient
ΔCCys221,222→ΔWild-type protein lacking COOH-terminal prenylation+
  • a β-Galactosidase activity was determined by filter assay. Plus (+) represents a positive and minus (−) a negative indication of β-galactosidase activity.