Table II.

Strain list

StrainGenotype
Y190MATa ura3-52 his3-Δ200 ade2-101 lys2-801 trp1-901 leu2-3,112 gal4-542 gal80-538 URA3::GAL-LacZ LYS2::GAL-HIS3 cyhr
NY10MATα ura3-52
NY26MATα ura3-52 sec2-59
NY603MATα leu2-3,112 ura3-52 pep4::URA3 Gal+
NY2146MATa sec2-Δ1::HIS3 leu2-3,112::[LEU2 SEC2-GFP] his3-Δ200 ura3-52 Gal+
NY2147MATa sec2-Δ1::HIS3 leu2-3,112::[LEU2 sec2-59-GFP] his3-Δ200 ura3-52 Gal+
NY2152MATa sec2-Δ1::HIS3 leu2-3,112::[LEU2 sec2-78-GFP] his3-Δ200 ura3-52 Gal+
NY2179MATα leu2-3,112 ura3-52 sec2-78 Gal+
NY2426MATα ura3-52::[URA3 GALp-YPT32; pNB1162] sec2-59
NY2427MATα leu2-3,112::[LEU2 GALp-YPT32; pNB831] ura3-52 sec2-78 Gal+
NY2428MATa sec2-Δ1::HIS3 leu2-3,112::[LEU2 sec2-59-GFP] his3-Δ200 ura3-52::[URA3 GALp-YPT32; pNB1162] Gal+
NY2429MATa sec2-Δ1::HIS3 leu2-3,112::[LEU2 sec2-78-GFP] his3-Δ200 ura3-52::[URA3 GALp-YPT32; pNB1162] Gal+
NY2430MATa sec2-Δ1::HIS3 leu2-3,112::[LEU2 sec2-78-GFP] his3-Δ200 ura3-52::[URA3 GALp-YPT31; pNB1178] Gal+
NY2431MATα ura3-52 sec2-78 + [GPDp-YPT32 2μ; pNB1163]
NY2432MATα leu2-3,112::[LEU2 GALp-GST-SEC2; pNB1153] ura3-52 pep4::URA3 Gal+
NY2433MATα leu2-3,112::[LEU2 GALp-GST; pNB1154] ura3-52 pep4::URA3 Gal+
NY2434MATα leu2-3,112::[LEU2 GALp-GST-sec2(aa.1-160); pNB1157] ura3-52 pep4::URA3 Gal+
NY2435MATα leu2-3,112::[LEU2 GALp-GST-sec2-59(aa.1-374); pNB1155] ura3-52 pep4::URA3 Gal+
NY2436MATα leu2-3,112::[LEU2 GALp-GST-sec2-70(aa.1-508); pNB1156] ura3-52 pep4::URA3 Gal+
NY2437MATα leu2-3,112::[LEU2 GALp-GST-sec2(aa.161-759); pNB1159] ura3-52 pep4::URA3 Gal+
NY2438MATα leu2-3,112::[LEU2 GALp-GST-sec2-78; pNB1160] ura3-52 pep4::URA3 Gal+
NY2439MATα leu2-3,112::[LEU2 GALp-GST-sec2(aa.1-450); pNB1161] ura3-52 pep4::URA3 Gal+
NY2440MATα leu2-3,112::[LEU2 GALp-YPT1; pNB829] ura3-52 sec2-78 Gal+
NY2441MATα leu2-3,112::[LEU2 GALp-SEC4; pNB833] ura3-52 sec2-78 Gal+
NY2442MATα ura3-52::[URA3 GALp-YPT1; pNB1176] sec2-59
NY2443MATα ura3-52::[URA3 GALp-SEC4; pNB1177] sec2-59
NY2444MATα ura3-52::[URA3 GALp-YPT31; pNB1178] sec2-59
NY2445MATα leu2-3,112 ura3-52::[URA3 GALp-YPT31; pNB1178] sec2-78 Gal+
NY2446MATa sec2-Δ1::HIS3 leu2-3,112::[LEU2 sec2-78-GFP] his3-Δ200 ura3-52::[URA3 GALp-SEC4; pNB1177] Gal+