Table I.

Targeting efficiencies and blebbing-inducing activities of the E4orf4-GFP proteins

Constructs293T
 TargetingC-33A
 TargetingC-33A
 Blebbing293
 Targeting293
 Blebbing
% cells
E4orf4-GFP N = 3561 N = 4239
E4orf4-GFP-NLS N = 94 N = 6518 N = 913
E4orf4-GFP-NESC = 92
E4orf4-GFP-CAAXM = 96M = 93 55M = 9276
Myr-GFP-E4orf4M = 33
n > 850n > 600n = 500n > 150
  • Transfection of the indicated GFP constructs was performed in 293T, 293, and C-33A, and the protein targeting efficiencies and blebbing-inducing activities (percentage blebbing) were determined by fluorescence microscopy in live cells 24 h after transfection; C, cytoplasmic; M, cell membranes; N, nuclear.