Table I.

List of strains

Strain nameRelevant genotype
FLY93MATα ura3-52 leu2-3,112 trp1Δ1 his3Δ200 (S288C genetic background)
FLY95MATα ura3-52 leu2-3,112 trp1Δ1 his3Δ200 (S288C genetic background)
FLY168MATα mob2Δ::HIS3
FLY757MATα cbk1Δ::kanMX
FLY782MATα cbk1Δ::kanMX mob2Δ::HIS3
FLY766MATα MOB2-GFP::HIS3 cbk1Δ::kanMX
FLY770MATα MYO1-GFP::kanMX mob2Δ::HIS3
FLY774MATα CBK1-GFP::kanMX mob2Δ::HIS3
FLY811MATα ACE2-GFP::kanMX
FLY823MATα MOB2-GFP::HIS3 bni1Δ::kanMX
FLY827MATα MOB2-GFP::HIS3 myo4Δ::kanMX
FLY841MATα ASH1-GFP::kanMX mob2Δ::HIS3
FLY850MATα ACE2-GFP::kanMX mob2Δ::HIS3
FLY856MATα ACE2-GFP::kanMX cbk1Δ::HIS3
FLY880MATα ace2Δ::kanMX
FLY893MATα/MATα MOB2-GFP::kanMX/ MOB2-GFP::HIS3
FLY895MATα/MATα CBK1-GFP::kanMX/CBK1-GFP::kanMX
FLY896MATα/MATα CBK1-CFP::kanMX/CBK1-CFP::kanMX HHF2-YFP::HIS3/HHF2-YFP::HIS3
FLY906MATα CBK1-6xMyc::kanMX mob2Δ::HIS3
FLY924MATα/MATα MOB2-CFP::kanMX/MOB2-CFP::kanMX HHF2-YFP::HIS3/HHF2-YFP::HIS3
FLY934MATα MOB2-GFP::HIS3 ace2Δ::kanMX
FLY954MATα CBK1-3xHA::HIS3 MOB2-6xMyc::kanMX
FLY960MATα CBK1-6xMyc::kanMX MOB2-3xHA::HIS3
FLY970MATα CBK1-GFP::kanMX ace2Δ::kanMX
FLY978MATα ACE2-GFP::kanMX mob1-7
FLY981MATα MOB2-GFP:kanMX mob1-77
FLY985*MATα ACE2-GFP::kanMX xpo1Δ::LEU2 [pKW440-XPO1-HIS3]
FLY986*MATα ACE2-GFP::kanMX xpo1Δ::LEU2 [pKW457-xpo1-1-HIS3]
FLY1005MATα MOB2-3xHA::HIS3 cbk1Δ::kanMX
FLY1007MATα cbk1Δ::kanMX [pRS316-cbk1D475A]
FLY1008MATα cbk1Δ::kanMX [pRS316-cbk1-as2]
FLY1009MATα ACE2-GFP::kanMX cbk1Δ::HIS3 [pRS316-cbk1-as2]
FLY1010MATα MOB2-GFP::kanMX cbk1Δ::HIS3 [pRS316-cbk1-as2]
FLY1011MATα MOB2-GFP::HIS3 cdc14-1
FLY1015MATα ACE2-GFP::HIS3 cdc14-1
KWY120MATα xpo1Δ::LEU2 ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 [pKW440-XPO1-HIS3]
KWY121MATα xpo1Δ::URA3 ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 [pKW457-xpo1-1-HIS3]
  • All strains are derived from S288C isolates FLY93 and FLY95, except those noted by *, which are derived from KWY120 and KWY121 (provided by K. Weis, University of California, Berkeley). Plasmids are bracketed.