Table III.

Cell cycle analysis using histone H2B-GFP

Wild-typeaCeCDC-14-RNAia,b
1st to 2nd mitotic metaphase17 min, 22 s ± 1 min, 45 s18 min, 13 s ± 2 min, 1 s
Nuclear envelope breakdown to 1st metaphase3 min, 6 s ± 19 s3 min, 7 s ± 24 s
1st mitotic metaphase to chromosome decondensation2 min, 25 s ± 11 s2 min, 32 s ± 22 s
  • a n = 7 for both wild-type and CeCDC-14–depleted embryos.

  • b For this experiment CeCDC-14 depleted embryos were used 30–36 h postinjection in order to ensure maximal depletion of the protein.