Table I.

Altering the activity of myosin, RhoA and MLCK does not alter growth cone F-actin content

TreatmentMean growth cone f-actin content
 in arbitrary units + SEM (n)
BSA (control)1263 ± 163 (30)
Skmyosin II1167 ± 142 (32)
MLCKp11192 ± 97 (43)
C31184 ± 171 (26)
CaRhoA1114 ± 158 (56)
  • Dorsal root ganglion neurons were trituration loaded with protein or peptide, cultured for 3 h and then fixed and stained with phalloidin. No statistically significant differences from control trituration loaded growth cones were observed.