Table I.

Rate and extent of FRAP aurora B–GFP or the kinase inactive mutant of aurora B, aurora B(K-R)-GFP

Slopet1/2Recoveryn
ks%
Aurora B–GFP
Prometaphase centromeres−0.017 ± 0.00747 ± 2478 ± 1313
Nocodazole centromeres−0.013 ± 0.00562 ± 1959 ± 1220
Aurora B(K-R)-GFP
Prometaphase centromeres−0.009 ± 0.00384 ± 3548 ± 1413
  • The rate constant k was measured as the negative slope of In(i − it) versus time after photobleaching. Halftime was calculated as t1/2 = ln2*(−1/k), as described in Materials and methods. Values are shown ± SD.