Table I.

Yeast strains used in this study

StrainGenotype
SBY3MATa ura3-1 leu2-3,112 his3-11 trp1-1 can1-100 ade2-1 bar1Δ
SBY97MATa ura3-1:TUB1-GFP:URA3 leu2-3,112 his3-11:pCUP1-GFP12-lacI12:HIS3 trp1-1:lacO:TRP1 lys2Δ ade2-1 bar1Δ can1-100 ipl1-321
SBY130MATa ura3-1:TUB1-GFP:URA3 leu2-3,112 his3-11:pCUP1-GFP12-lacI12:HIS3 trp1-1:lacO:TRP1 lys2Δ ade2-1 bar1Δ can1-100
SBY322MATa ura3-1 leu2-3,112 his3-11:pCUP1-GFP12-lacI12:HIS3 trp1-1:lacO:TRP1 lys2Δade2-1 bar1Δ can1- 100 ipl1-321
SBY539MATa ura3-1 leu2-3,112 his3-11 trp1-1 can1-100 ade2-1 bar1Δ NDC10-GFP:KAN
SBY554MATa ura3-1:pGAL-IPL1:URA3 leu2-3,112 his3-11 trp1-1 can1-100 ade2-1 bar1Δ
SBY556MATa ura3-1 leu2-3,112 his3-11 trp1-1 ade2-1 bar1Δ can1-100 IPL1-GFP:KAN
SBY617MATa ura3-1 leu2-3,112 his3-11 trp1-1 ade2-1 bar1Δ can1-100 CSE4-myc12:URA3
SBY736MATa ura3-1:pGAL-myc13-IPL1:URA3 leu2-3,112 his3-11 trp1-1 can1-100 ade2-1 bar1Δ
SBY875MATa ura3-1 leu2-3,112 his3-11 trp1-1 can1-100 ade2-1 bar1Δ SLI15-GFP:HIS3
SBY943MATa ura3-1:TUB1-GFP:URA3 leu2-3,112 his3-11 trp1-1 ade2-1 can1-100 cdc20::LEU2 ipl1-321 [pGAL(low)-CDC20-HIS3(CEN)]
SBY952MATa ura3-1:TUB1-GFP:URA3 leu2-3,112 his3-11 trp1-1 ade2-1 can1-100 cdc20::LEU2 (pGAL[low]- CDC20-HIS3[CEN])
SBY965MATa ura3-1 leu2-3,112:TUB1-GFP:LEU2 his3-11:pCUP1-GFP12-lacI12:HIS3 trp1-1:lacO:URA3:TRP1 lys2Δ ade2-1 mdc1-1 cdc26::KAN
SBY1036MATa ura3-1:TUB1-CFP:URA3 leu2-3,112 his3-11 trp1-1 ade2-1 bar1Δ can1-100 IPL1-GFP:KAN
SBY1115MATα ura3-1 leu2-3,112 his3-11 trp1-1 can1-100 ade2-1 bar1Δ DAM1-GFP:TRP1
SBY1246MATα ura3-1 leu2-3,112 his3-11,15 trp1-1 ade2-1 can1-100 cdc20::LEU2 IPL1-YFP:HIS3 NDC10-CFP: KAN (pGAL[low]-CDC20-HIS3[CEN])
SBY1422MATa ura3-1:TUB1-GFP:URA3 leu2-3,112 his3-11:pCUP1-GFP12-lacI12:HIS3 trp1-1:lacO:TRP1 lys2Δ ade2-1 bar1Δ can1-100 mad2::KAN
SBY1423MATa ura3-1:TUB1-GFP:URA3 leu2-3,112 his3-11:pCUP1-GFP12-lacI12:HIS3 trp1-1:lacO:TRP1 lys2Δ ade2-1 bar1Δ can1-100 mad1::KAN
SBY2066MATa leu2-3,112 his3-11:LacI12:HIS3 trp1-1:LacO:TRP1 lys2Δ bar1Δ ipl1-321 mcd1-1 cdc26::KAN
  • All strains are isogenic with the W303 strain background. Plasmids are indicated in parentheses.