Table I.

Time spent in prometaphase by PtK1 cells treated with Hesperadin

PtK1 cellsNEB to last chromosome congressingNEB to anaphase onsetNo. of monooriented chromosomes at anaphase onsetn
min:secmin:sec
Controla13:19 (08:30–21:14)23:12 (14:00–50:45)016
HesperadinaNA16:27 (10:30–23:46)3.6 (1–6)23
  • a PtK1 cells were left untreated or were treated with 500 nM Hesperadin, and followed by video microscopy in a 37°C warm room.