Table III.

Mutations in lst8 cause increased Gln3p-dependent transcription

StrainPGAP1-LacZ expression in glutamine mediumStandard deviation
(β-galactosidase units)(β-galactosidase units)
WT12.70.4
lst8-135.95.8
lst8-614.61.5
lst8-731.32.5
mks1Δ0.40.1
ure2Δ361.478.9
tor1Δ24.66.0
WT + 200 ng/ml rap (1 h)25.11.5
WT + 200 ng/ml rap (2 h)82.22.3
  • Strains were as listed in Table II and tor1Δ ura3-52 (CKY794), containing pMS29.